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Literature summary for 3.2.1.14 extracted from

  • Arakane, Y.; Zhu, Q.; Matsumiya, M.; Muthukrishnan, S.; Kramer, K.J.
    Properties of catalytic, linker and chitin-binding domains of insect chitinase (2003), Insect Biochem. Mol. Biol., 33, 631-648.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Hi-5 insect cells using baculovirus vectors Manduca sexta

Protein Variants

Protein Variants Comment Organism
DELTA1-376 ChiLCH, with a C-terminal His tag Manduca sexta
DELTA1-477 ChiCH, with a C-terminal His tag Manduca sexta
DELTA1-79 Chi80-535, enzymatically inactive Manduca sexta
DELTA377-535 Chi376, no secretion into the medium Manduca sexta
DELTA387-477 Chi386(MCBD) Manduca sexta
DELTA387-535 Chi386 Manduca sexta
DELTA397-535 Chi396 Manduca sexta
DELTA408-535 Chi407 Manduca sexta
DELTA478-535 Chi477 Manduca sexta
additional information Chi386(MCBD)2: Chi386 protein fused with doubbled amino acid residues 478-535. Chi386(RCBD): Chi386 protein fused with amino acid residues 19-68 of a rice class I chitinase. ChiLH: linker region consisting of amino acid residues 377-477 with a C-terminal His tag, no secretion into the medium Manduca sexta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the KM-values with chitin as substrate are for wild-type: 4.29 mg/ml, Chi477: 9.96 mg/ml, Chi396: 20 mg/ml, Chi386: 29 mg/ml, Chi386(MCBD): 4.98 mg/ml, Chi386(MCBD)2: 4.67 mg/ml and Chi386(RCBD): 3.18 mg/ml Manduca sexta
0.29
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD) Manduca sexta
0.31
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD)2 Manduca sexta
0.48
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(RCBD) Manduca sexta
0.51
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi477 Manduca sexta
0.64
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi396 Manduca sexta
0.93
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386 Manduca sexta
1.13
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, wild-type Manduca sexta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 x * 79000, SDS-PAGE Manduca sexta

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
 tobacco hornworm
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylated with mannose and N-acetylglucosamine as most abundant sugars, glucose and galactose Manduca sexta

Purification (Commentary)

Purification (Comment) Organism
-
 Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Manduca sexta ?
-
 ?
chitin + H2O
-
 Manduca sexta ?
-
 ?

Subunits

Subunits Comment Organism
? x * 79000, SDS-PAGE Manduca sexta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD) Manduca sexta
0.17
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD)2 Manduca sexta
0.22
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(RCBD) Manduca sexta
0.27
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi396 Manduca sexta
0.31
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi477 Manduca sexta
0.37
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386 Manduca sexta
0.76
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, wild-type Manduca sexta