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Literature summary for 3.2.1.14 extracted from

  • Chang, M.C.; Lai, P.L.; Wu, M.L.
    Biochemical characterization and site-directed mutational analysis of the double chitin-binding domain from chitinase 92 of Aeromonas hydrophila JP101 (2004), FEMS Microbiol. Lett., 232, 61-66.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 with a N-terminal T7 tag and a C-terminal His tag Aeromonas hydrophila

Protein Variants

Protein Variants Comment Organism
W773A mutation in a chitin-binding domain, much weaker affinity for chitin than wild-type Aeromonas hydrophila
W792A mutation in a chitin-binding domain, much weaker affinity for chitin than wild-type Aeromonas hydrophila
W797A mutation in a chitin-binding domain, much weaker affinity for chitin than wild-type Aeromonas hydrophila
Y796A mutation in a chitin-binding domain, much weaker affinity for chitin than wild-type Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
JP101
-
Aeromonas hydrophila JP101
-
JP101
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeromonas hydrophila

Synonyms

Synonyms Comment Organism
chitinase 92
-
Aeromonas hydrophila