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Literature summary for 3.2.1.14 extracted from
- An extracellular Bacillus sp. chitinase for the production of chitotriose as a major chitinolytic product (2003), Biotechnol. Lett., 25, 409-412.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM for colloidal chitin is 3 mg/ml | Bacillus sp. (in: Bacteria) |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
Wy22 | - |
| Bacillus sp. (in: Bacteria) Wy22 | - |
Wy22 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
after Sephadex G200 purification step | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | colloidal and swollen chitin | Bacillus sp. (in: Bacteria) | chitotriose + ? | a major product from colloidal chitin | ? |  |
| chitin + H2O | colloidal and swollen chitin | Bacillus sp. (in: Bacteria) Wy22 | chitotriose + ? | a major product from colloidal chitin | ? | |
| chitosan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| chitosan + H2O | - |
Bacillus sp. (in: Bacteria) Wy22 | ? | - |
? | |
| glycol chitin + H2O | - |
Bacillus sp. (in: Bacteria) | N,N',N''-triacetylchitotriose + ? | a major product | ? | |
| glycol chitin + H2O | - |
Bacillus sp. (in: Bacteria) Wy22 | N,N',N''-triacetylchitotriose + ? | a major product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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