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Literature summary for 3.2.1.14 extracted from
- Purification, characterization, and molecular cloning of a chitinase from the seeds of Benincasa hispida (2001), Biosci. Biotechnol. Biochem., 65, 501-509.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Benincasa hispida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 29000, PAGE and SDS-PAGE | Benincasa hispida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Benincasa hispida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Benincasa hispida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Benincasa hispida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6688 | - |
after CM-Sepharose column purification step | Benincasa hispida |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, PAGE and SDS-PAGE | Benincasa hispida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
70 | stable for 1 h | Benincasa hispida |
| 80 | - |
total loss of activity after 1 h | Benincasa hispida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
Benincasa hispida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | decreasing of activity from pH 2 to pH 8 | Benincasa hispida |
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