Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Watanabe, T.; Ariga, Y.; Sato, U.; Toratani, T.; Hashimoto, M.; Nikaidou, N.; Kezuka, Y.; Nonaka, T.; Sugiyama, J.
    Aromatic residues within the substrate-binding cleft of Bacillus circulans chitinase A1 are essential for hydrolysis of crystalline chitin (2003), Biochem. J., 376, 237-244.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Niallia circulans

Protein Variants

Protein Variants Comment Organism
W164A decreased hydrolysing activity against highly crystalline beta-chitin and colloidal chitin, but enhanced activities against soluble substrates Niallia circulans
W164A/W285A drastically decreased activity against all substrates Niallia circulans
W285A decreased hydrolysing activity against highly crystalline beta-chitin and colloidal chitin, but enhanced activities against soluble substrates Niallia circulans
W433A drastically decreased activity against all substrates Niallia circulans
W53A decreased hydrolysing activity against highly crystalline beta-chitin Niallia circulans
Y279F drastically decreased activity against all substrates Niallia circulans
Y56A decreased hydrolysing activity against highly crystalline beta-chitin Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, Y279F Niallia circulans
0.002
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, wild-type Niallia circulans
0.003
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, W433A Niallia circulans
0.081
-
 reduced chitopentaose 37°C, pH 6, wild-type Niallia circulans
0.177
-
 reduced chitopentaose 37°C, pH 6, W285A Niallia circulans
0.206
-
 reduced chitopentaose 37°C, pH 6, W164A Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-tri-N-acetylchitotriose + H2O
-
 Niallia circulans ?
-
 ?
beta-chitin + H2O
-
 Niallia circulans ?
-
 ?
carboxymethyl chitin + H2O
-
 Niallia circulans ?
-
 ?
colloidal chitin + H2O
-
 Niallia circulans ?
-
 ?
reduced chitopentaose + H2O
-
 Niallia circulans ?
-
 ?

Synonyms

Synonyms Comment Organism
ChiA1
-
 Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, W433A Niallia circulans
0.14
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, Y279F Niallia circulans
9.55
-
 carboxymethyl chitin 37°C, pH 6, wild-type Niallia circulans
22.4
-
 4-methylumbelliferyl-tri-N-acetylchitotriose 37°C, pH 6, wild-type Niallia circulans
24.1
-
 carboxymethyl chitin 37°C, pH 6, W164A Niallia circulans
25.2
-
 carboxymethyl chitin 37°C, pH 6, W285A Niallia circulans
26.9
-
 reduced chitopentaose 37°C, pH 6, wild-type Niallia circulans
35.6
-
 reduced chitopentaose 37°C, pH 6, W164A Niallia circulans
45.1
-
 reduced chitopentaose 37°C, pH 6, W285A Niallia circulans