KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | colloidal chitin, chit33 0.3 mg/ml, chit37 0.5 mg/ml, chit42 1 mg/ml | Trichoderma harzianum | |
0.7 | - |
p-nitrophenyl beta-D-N,N'-diacetylchitobiose | chit37 | Trichoderma harzianum | |
0.85 | - |
p-nitrophenyl beta-D-N,N'-diacetylchitobiose | chit42 | Trichoderma harzianum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
chit33, gel filtration and SDS-PAGE | Trichoderma harzianum |
37000 | - |
chit37, gel filtration and SDS-PAGE | Trichoderma harzianum |
42000 | - |
chit42, gel filtration and SDS-PAGE | Trichoderma harzianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
CECT 2413 | - |
Trichoderma harzianum CECT 2413 | - |
CECT 2413 | - |
Purification (Comment) | Organism |
---|---|
chit33 1.3fold to homogeneity, chit42 8fold to homogeneity, chit37 3.4fold to homogeneity | Trichoderma harzianum |
Renatured (Comment) | Organism |
---|---|
- |
Trichoderma harzianum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Trichoderma harzianum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
chit33 | Trichoderma harzianum |
2.78 | - |
chit37 | Trichoderma harzianum |
6.54 | - |
chit42 | Trichoderma harzianum |
Storage Stability | Organism |
---|---|
4°C, stable for at least one month | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O | chit37 and chit42 | Trichoderma harzianum | 4-methylumbelliferone + N,N'-diacetylchitobiose | - |
? | |
cell wall + H2O | chit42, cell wall from Botrytis cinerea | Trichoderma harzianum | chitin oligosaccharides | - |
? | |
chitin + H2O | colloidal chitin | Trichoderma harzianum | N,N-diacetylchitobiose + N-acetylglucosamine | chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present | ? | |
chitin + H2O | colloidal chitin | Trichoderma harzianum CECT 2413 | N,N-diacetylchitobiose + N-acetylglucosamine | chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present | ? | |
chitopentaose + H2O | - |
Trichoderma harzianum | chitobiose + chitotriose | main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also | ? | |
chitopentaose + H2O | - |
Trichoderma harzianum CECT 2413 | chitobiose + chitotriose | main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also | ? | |
glycol chitin + H2O | - |
Trichoderma harzianum | N-acetylglucosamine + ? | - |
? | |
glycol chitin + H2O | - |
Trichoderma harzianum CECT 2413 | N-acetylglucosamine + ? | - |
? | |
glycol chitosan + H2O | chit37 and chit42 | Trichoderma harzianum | ? | - |
? | |
glycol chitosan + H2O | chit37 and chit42 | Trichoderma harzianum CECT 2413 | ? | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Trichoderma harzianum | N-acetylglucosamine + N,N'-diacetylchitobiose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Trichoderma harzianum CECT 2413 | N-acetylglucosamine + N,N'-diacetylchitobiose | - |
? | |
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O | - |
Trichoderma harzianum | p-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O | chit37 and chit42 | Trichoderma harzianum | N,N'-diacetylchitobiose + p-nitrophenol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | chit42 | Trichoderma harzianum |
45 | 50 | chit33 and chit37 | Trichoderma harzianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable below 50°C, chit42 | Trichoderma harzianum |
60 | - |
stable below 60°C, chit33 and chit37 | Trichoderma harzianum |