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Literature summary for 3.2.1.14 extracted from

  • Mahadevan, B.; Crawford, D.L.
    Properties of the chitinase of the antifungal biocontrol agent Strptomyces lydicus WYEC108 (1997), Enzyme Microb. Technol., 20, 489-493.
No PubMed abstract available
Search for more literature for 3.2.1.14

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces lydicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000 + x * 37000, SDS-PAGE Streptomyces lydicus
37000
-
 x * 32000 + x * 37000, SDS-PAGE Streptomyces lydicus

Organism

Organism UniProt Comment Textmining
Streptomyces lydicus
-
 WYEC108
-
Streptomyces lydicus WYEC108
-
 WYEC108
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lydicus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces lydicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces lydicus

Storage Stability

Storage Stability Organism
4°C, purified enzyme, more than six months, no loss of activity Streptomyces lydicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall + H2O fungal, Phytium and Rhizoctonia solani Streptomyces lydicus chitin oligosaccharides N-acetylglucosamine only ?
cell wall + H2O fungal, Phytium and Rhizoctonia solani Streptomyces lydicus WYEC108 chitin oligosaccharides N-acetylglucosamine only ?
chitin + H2O colloidal chitin Streptomyces lydicus N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?
chitin + H2O colloidal chitin Streptomyces lydicus WYEC108 N,N-diacetylchitobiose + N-acetylglucosamine N-acetylglucosamine only ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Streptomyces lydicus N,N'-diacetylchitobiose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Streptomyces lydicus WYEC108 N,N'-diacetylchitobiose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 32000 + x * 37000, SDS-PAGE Streptomyces lydicus