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Literature summary for 3.2.1.14 extracted from
- Bovine serum chitinase (1979), Eur. J. Biochem., 100, 455-460.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | small amounts, pH 6.4 | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Bos taurus |  |
| Trypsin | small amounts, pH more than 7.4 | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | glycol chitin 0.76 mg/ml | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | Na+ restores activity after EDTA treatment | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1000fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
60000 Hultin Units/mg | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Bos taurus | N,N-diacetylchitobiose + N-acetylglucosamine | - |
? | |
| glycol chitin + H2O | - |
Bos taurus | N-acetylglucosamine + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at pH 1.5 | Bos taurus |
| 51 | - |
at pH 3.5 | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
activity decreases | Bos taurus |
| 65 | - |
70% loss of activity after 15 min | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 2 | glycol chitin | Bos taurus |
| 3 | 6 | colloidal chitin | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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