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Literature summary for 3.2.1.14 extracted from
- Chitinolytic activity from Neurospora crassa (1983), J. Gen. Microbiol., 129, 3319-3326.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FeCl3 | 15% increas in activity at 10 mM | Neurospora crassa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Neurospora crassa | |
| HgCl2 | - |
Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20600 | - |
gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7.7fold | Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C or below, several weeks, no loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | nascent more effectively than preformed chitin | Neurospora crassa | N,N-diacetylchitobiose + N-acetylglucosamine | N-N'-diacetylchitobiose main product, also chitotriose and chitotetraose, with preformed chitin as substrate also N-acetylglucosamine | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 92 | - |
75% loss of activity after 30 min at 92°C | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Neurospora crassa |
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