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Literature summary for 3.2.1.14 extracted from
- Chitinase from Verticillum albo-atrum (1988), Methods Enzymol., 161, 474-479.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Verticillium albo-atrum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Acetamido-2-deoxy-D-gluconolactone | - |
Verticillium albo-atrum | |
| CuSO4 | - |
Verticillium albo-atrum | |
| EDTA | - |
Verticillium albo-atrum | |
| HgCl2 | - |
Verticillium albo-atrum | |
| KCN | - |
Verticillium albo-atrum | |
| LKB ampholytes | - |
Verticillium albo-atrum | |
| MnSO4 | - |
Verticillium albo-atrum | |
| ZnSO4 | - |
Verticillium albo-atrum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | chitin, 97 mg dry weight per ml | Verticillium albo-atrum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Verticillium albo-atrum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Verticillium albo-atrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, SDS-PAGE | Verticillium albo-atrum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Verticillium albo-atrum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Verticillium albo-atrum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Verticillium albo-atrum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, several months, no loss of activity | Verticillium albo-atrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
| chitin + H2O | from ground crab shell | Verticillium albo-atrum | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylglucosamine only | ? | |
| chitosan + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | - |
Verticillium albo-atrum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Verticillium albo-atrum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
- |
Verticillium albo-atrum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
enzyme inactivated at 70°C | Verticillium albo-atrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
- |
Verticillium albo-atrum |
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Release 2023.1 (January 2023)
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