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Literature summary for 3.11.1.2 extracted from
- Phosphonoacetate hydrolase from Penicillium oxalicum: Purification and properties, phosphate starvation-independent expression, and partial sequencing (2006), Res. Microbiol., 157, 125-135.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
Phosphonoacetate | pH 8.0, 35°C | Penicillium oxalicum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42600 | - |
gel filtration | Penicillium oxalicum |
| 43200 | - |
1 * 43200, SDS-PAGE | Penicillium oxalicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium oxalicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 665 | - |
pH 8.0, 35°C | Penicillium oxalicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Phosphonoacetate + H2O | - |
Penicillium oxalicum | acetate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43200, SDS-PAGE | Penicillium oxalicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
- |
Penicillium oxalicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
Phosphonoacetate | pH 8.0, 35°C | Penicillium oxalicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Penicillium oxalicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.6 | more than 90% of maximum activity | Penicillium oxalicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
above pH 6.2 stable for at least 10 min | Penicillium oxalicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium oxalicum | isoelectric focusing | - |
7.1 |
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Release 2023.1 (January 2023)
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