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Literature summary for 3.10.1.1 extracted from
- Recombinant human sulphamidase: expression, amplification, purification and characterization (1998), Biochem. J., 329, 145-150.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme-replacement or gene-replacement therapy | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Chinese hamster ovary cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0107 | - |
tetrasaccharides | substrates deriving from heparan sulfate, unamplified recombinant enzyme | Homo sapiens | |
| 0.01225 | - |
tetrasaccharides | substrates deriving from heparan sulfate, gene-amplified recombinant enzyme | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54680 | - |
calculation from cDNA sequence | Homo sapiens |
| 57000 | - |
SDS-PAGE, mature enzyme | Homo sapiens |
| 62000 | - |
2 * 62000, SDS-PAGE | Homo sapiens |
| 63000 | - |
SDS-PAGE, precursor of recombinant enzyme | Homo sapiens |
| 115000 | - |
gel filtration, Superose 12 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate + H2O | Homo sapiens | - |
? + sulfate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
? | |
| heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
ir | |
| tetrasaccharides + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
recombinant enzyme | Homo sapiens |
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