Literature summary for 3.1.7.2 extracted from

Sun, D.; Lee, G.; Lee, J.H.; Kim, H.Y.; Rhee, H.W.; Park, S.Y.; Kim, K.J.; Kim, Y.; Kim, B.Y.; Hong, J.I.; Park, C.; Choy, H.E.; Kim, J.H.; Jeon, Y.H.; Chung, J.
A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses (2010), Nat. Struct. Mol. Biol., 17, 1188-1194.

Search for more literature for 3.1.7.2

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells	Homo sapiens
recombinant expression of full-length enzyme in Escherichia coli and in S2 cells	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
additional information	Mesh1 deletion by imprecise excision of a P-element from the Drosophila Mesh1 G3858 allele, resulting in the Drosophila Mesh1 null allele, Mesh1 5A3. The Mesh1 null mutant is much more susceptible than wild-type flies to death from amino acid starvation, and this susceptibility is also completely rescued by Mesh1 expression	Drosophila melanogaster

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates, but less effective than Mn2+	Drosophila melanogaster
Mn2+	activates, required	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1	?	-	?
ppGpp + H2O	Drosophila melanogaster	-	ppG + diphosphate	-	?
ppGpp + H2O	Homo sapiens	-	ppG + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant full-length enzyme from Escherichia coli	Drosophila melanogaster
recombinant full-length enzyme from Escherichia coli	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1	Homo sapiens	?	-	?
additional information	activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview	Drosophila melanogaster	?	-	?
additional information	activity detection by usage of the chemosensor pyrene and bis(Zn2+-dipicolylamine), which generates fluorescence at 470 nm when it specifically binds to ppGpp, overview	Homo sapiens	?	-	?
ppGpp + H2O	-	Drosophila melanogaster	ppG + diphosphate	-	?
ppGpp + H2O	-	Homo sapiens	ppG + diphosphate	-	?

Subunits

Subunits	Comment	Organism
More	MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview	Drosophila melanogaster
More	MESH1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains, structure comparisons, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
HDDC3	-	Homo sapiens
MESH1	-	Drosophila melanogaster
MESH1	-	Homo sapiens
metazoan SpoT homolog-1	Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains	Drosophila melanogaster
metazoan SpoT homolog-1	Mesh1 consists only of the hydrolase domain of bacterial SpoT and lack the synthetase and regulatory domains	Homo sapiens
ppGpp hydrolase	-	Drosophila melanogaster
ppGpp hydrolase	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	Mesh1 deletion impairs starvation resistance in Drosophila, overview	Drosophila melanogaster
additional information	crucial residues for the ppGpp hydrolysis activity of Mesh1 are Arg24, Glu65, Asp66 and Asn126	Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9.46	-	ppGpp	pH not specified in the publication, temperature not specified in the publication	Homo sapiens
9.58	-	ppGpp	pH not specified in the publication, temperature not specified in the publication	Drosophila melanogaster

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Release 2023.1 (January 2023)