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Literature summary for 3.1.4.17 extracted from

  • Sharma, R.K.
    Diversity of calcium action in regulation of mammalian calmodulin-dependent cyclic nucleotide phosphodiesterase (2003), Indian J. Biochem. Biophys., 40, 77-91.
    View publication on PubMed
Search for more literature for 3.1.4.17

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin differential effect on isozymes, activation only in complex with Ca2+, overview Mammalia
Calmodulin differential effect on isozymes, activation only in complex with Ca2+, overview Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
3-isobutyl-1-methylxanthine marked differences in inhibition for isozymes Bos taurus
amantadine brain 60 kDa isozyme Bos taurus
angiotensin III
-
 Bos taurus
felodipine
-
 Bos taurus
nicardipine
-
 Bos taurus
sarilesin
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 cGMP brain 63 kDA isozyme Bos taurus
0.0027
-
 cGMP brain 60 kDA isozyme Bos taurus
0.0028
-
 cGMP lung isozyme Bos taurus
0.0032
-
 cGMP heart isozyme Bos taurus
0.012
-
 cAMP brain 63 kDA isozyme Bos taurus
0.035
-
 cAMP brain 60 kDA isozyme Bos taurus
0.04
-
 cAMP heart isozyme Bos taurus
0.042
-
 cAMP lung isozyme Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol mainly Mammalia 5829
-
cytosol mainly Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ differential effect on isozymes, activation only in complex with calimodulin, overeview Mammalia
Ca2+ differential effect on isozymes, activation only in complex with calimodulin, overeview Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 overview Mammalia
60000
-
 2 * 60000, SDS-PAGE, brain PDE1 isoform Bos taurus
116000 124000 sedimentation data Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mammalia overview on physiological role ?
-
 ?
additional information Bos taurus overview on physiological role ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Mammalia
-
 overview on PDE1 isozymes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein 60 kDa PDE1, substrate of cAMP-dependent protein kinase, 63 kDa PDE1, substrate of calmodulin-dependent protein kinase, reversion for both by calcineurin, overview on regulation by phosphorylation Mammalia
phosphoprotein regulation by phosphorylation, overview Bos taurus

Purification (Commentary)

Purification (Comment) Organism
overview Mammalia
overview Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain highest enzymic activity Bos taurus
-
heart 10-20% of activity found in brain Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 kinetic properties, overview Mammalia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cAMP + H2O
-
 Bos taurus AMP
-
 ?
cGMP + H2O
-
 Bos taurus GMP
-
 ?
additional information overview on physiological role Mammalia ?
-
 ?
additional information overview on physiological role Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE, brain PDE1 isoform Bos taurus
More overview on subunit composition and partial amino acid sequences Mammalia
More PDE1 exists as tissue-specific and immunological distinct isozymes, overview Bos taurus