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Literature summary for 3.1.4.16 extracted from

  • Kimura, Y.; Okazaki, N.; Takegawa, K.
    Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3',5'- and 2'3'-cAMP phosphodiesterase, and phosphatase activities (2009), FEBS Lett., 583, 443-448.
    View publication on PubMed
Search for more literature for 3.1.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
phosphate PdeB is strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0) Myxococcus xanthus
Zn2+ 28% relative activity at 0.05 mM Zn2+, using cyclic 3',5'-AMP as substrate Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 cyclic 2',3'-AMP in 50 mM Tris-HCl, pH 8.0 Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ PdeB is stimulated about 2fold by 0.05 mM Co2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate Myxococcus xanthus
Mn2+ PdeB is stimulated about 2fold by 0.05 mM Mn2+, at pH 8.0 in 50 mM Tris-HCl buffer with cyclic 3',5'-AMP as substrate Myxococcus xanthus
additional information enzymatic activity is not stimulated by Ca2+, Mg2+, Fe2+, Fe3+, and Zn2+ Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon affinity column chromatography Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-AMP + H2O
-
 Myxococcus xanthus 3'-AMP
-
 r
additional information PdeB also shows high phosphomonoesterase activity against 5'-UMP, 3'-AMP, 3'-GMP, and low activity against 5'-dAMP Myxococcus xanthus ?
-
 ?
additional information the enzyme shows no activity toward 2'-AMP and 2'-GMP, 3',5'-cGMP is either not hydrolysed or is cleaved very slowly by PdeB Myxococcus xanthus ?
-
 ?

Synonyms

Synonyms Comment Organism
2',3'-cAMP phosphodiesterase
-
 Myxococcus xanthus
PdeB
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Myxococcus xanthus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55 heat treatment at 50 or 55°C for 5 min slightly activates the phosphodiesterase activity of PdeB against 3',5'-cAMP by about 1.2fold Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 cyclic 2',3'-AMP in 50 mM Tris-HCl, pH 8.0 Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 PdeB has slightly alkaline pH optimum (pH 7.5-8.5) for 3',5'-cAMP with 0.1 M Tris-HCl buffer Myxococcus xanthus