General Stability | Organism |
---|---|
chymotrypsin, 10 min at 37°C, 80% loss of phosphodiesterase activity and 3'-nucleotidase activity | Proteus mirabilis |
trypsin, 10 min at 37°C, 50% loss of phosphodiesterase activity | Proteus mirabilis |
urea, 6.4 M, 53% loss of activity after 10 min, 20% loss of activity after 20 min | Proteus mirabilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Proteus mirabilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCN | slight activation of cyclic phosphodiesterase | Proteus mirabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Proteus mirabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | - |
Proteus mirabilis | adenosine + phosphate | - |
? | |
cyclic 2',3'-CMP + H2O | - |
Proteus mirabilis | 3'-CMP | - |
? | |
cyclic 2',3'-GMP + H2O | - |
Proteus mirabilis | 3'-GMP | - |
? | |
cyclic 2',3'-UMP + H2O | - |
Proteus mirabilis | 3'-UMP | - |
? | |
di-p-nitrophenyl phosphate + H2O | - |
Proteus mirabilis | p-nitrophenol + p-nitrophenyl phosphate | - |
? | |
additional information | 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein | Proteus mirabilis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Proteus mirabilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 55% of maximal activity at pH 6 and at pH 9 | Proteus mirabilis |