Cloned (Comment) | Organism |
---|---|
- |
Chromobacterium violaceum |
Crystallization (Comment) | Organism |
---|---|
to 1.9 resolution, with AMP and orthophosphate bound in the active site. The enzyme has a trinuclear metal center in the active site with three Mn2+ ions. It belongs to the Cluster of Orthologous Groups cog0613 from the polymerase and histidinol phosphatase family of enzymes | Chromobacterium violaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
adenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0016 | - |
5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0052 | - |
2'-deoxyguanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0054 | - |
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0088 | - |
uridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0093 | - |
thymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0102 | - |
2'-deoxyadenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.0173 | - |
guanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.022 | - |
2'-deoxythymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.026 | - |
2'-deoxycytidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
0.029 | - |
2'-deoxyuridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enzyme has a trinuclear metal center in the active site with three Mn2+ ions | Chromobacterium violaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | Q7NXD4 | - |
- |
Chromobacterium violaceum DSM 30191 | Q7NXD4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxyadenosine 5'-phosphate + phosphate | - |
? | |
2'-deoxyadenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum DSM 30191 | 2'-deoxyadenosine 5'-phosphate + phosphate | - |
? | |
2'-deoxycytidine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxycytidine 5'-phosphate + phosphate | - |
? | |
2'-deoxycytidine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum DSM 30191 | 2'-deoxycytidine 5'-phosphate + phosphate | - |
? | |
2'-deoxyguanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxyguanosine 5'-phosphate + phosphate | - |
? | |
2'-deoxythymidine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxythymidine 5'-phosphate + phosphate | - |
? | |
2'-deoxyuridine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | 2'-deoxyuridine 5'-phosphate + phosphate | - |
? | |
5'-phosphoadenylyl-(3'->5')-adenosine + H2O | - |
Chromobacterium violaceum | ? + phosphate | - |
? | |
adenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | adenosine 5'-phosphate + phosphate | - |
? | |
adenosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum DSM 30191 | adenosine 5'-phosphate + phosphate | - |
? | |
cytidine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | cytidine 5'-phosphate + phosphate | - |
? | |
guanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | guanosine 5'-phosphate + phosphate | - |
? | |
guanosine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum DSM 30191 | guanosine 5'-phosphate + phosphate | - |
? | |
additional information | enzyme is promiscuous and is able to hydrolyze several 3',5'-bisphosphonucleotides such as pGp, pCp, pUp, and pIp, and 2'-deoxynucleotides with comparable catalytic efficiency. In addition, it is capable of hydrolyzing short oligonucleotides such as (pdA)5, albeit at rates much lower than that of adenosine 3',5'-bisphosphate | Chromobacterium violaceum | ? | - |
? | |
additional information | enzyme is promiscuous and is able to hydrolyze several 3',5'-bisphosphonucleotides such as pGp, pCp, pUp, and pIp, and 2'-deoxynucleotides with comparable catalytic efficiency. In addition, it is capable of hydrolyzing short oligonucleotides such as (pdA)5, albeit at rates much lower than that of adenosine 3',5'-bisphosphate | Chromobacterium violaceum DSM 30191 | ? | - |
? | |
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine + H2O | - |
Chromobacterium violaceum | ? + phosphate | - |
? | |
thymidine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | thymidine 5'-phosphate + phosphate | - |
? | |
uridine 3',5'-bisphosphate + H2O | - |
Chromobacterium violaceum | uridine 5'-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cv1693 | - |
Chromobacterium violaceum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum | |
3.3 | - |
thymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
4.2 | - |
2'-deoxyguanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
4.3 | - |
2'-deoxythymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
5.2 | - |
2'-deoxyuridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
5.4 | - |
uridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
7.1 | - |
2'-deoxyadenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
9.4 | - |
2'-deoxycytidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
11.6 | - |
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum | |
12.3 | - |
guanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
22 | - |
adenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum | |
180 | - |
2'-deoxyuridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
190 | - |
2'-deoxythymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
360 | - |
thymidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
360 | - |
2'-deoxycytidine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
610 | - |
uridine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
700 | - |
2'-deoxyadenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
710 | - |
guanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
790 | - |
2'-deoxyguanosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
1400 | - |
adenosine 3',5'-bisphosphate | pH 7.5, 3°C | Chromobacterium violaceum | |
2100 | - |
N6-methyl-5'-phosphoadenylyl-(3'->5')-adenosine | pH 7.5, 3°C | Chromobacterium violaceum |