Cloned (Comment) | Organism |
---|---|
expression of Yop51 and Yop51*, a C235R point mutation and expression of the catalytic domain, residues 163-468 in Escherichia coli | Yersinia enterocolitica |
Protein Variants | Comment | Organism |
---|---|---|
C235R | indistinguishable from wild-type, the truncated enzyme YOP*DELTA162 has no altered catalytic properties | Yersinia enterocolitica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | 57.7 | 4-nitrophenyl phosphate | value strongly dependent on ionic strength and pH | Yersinia enterocolitica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Yersinia enterocolitica | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
enzyme Yop51*Delta162, catalytic domain, gel filtration | Yersinia enterocolitica |
49800 | - |
enzymes Yop51, Yop51*, sedimentation equilibrium centrifugation | Yersinia enterocolitica |
51000 | - |
1 * 51000, enzymes Yop51 and Yop51*, SDS-PAGE | Yersinia enterocolitica |
74000 | - |
enzymes Yop51, Yop51*, gel filtration | Yersinia enterocolitica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia enterocolitica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Yersinia enterocolitica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate | mechanism | Yersinia enterocolitica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
799 | - |
enzyme Yop51 | Yersinia enterocolitica |
806 | - |
enzyme Yop51*, point mutation C235R | Yersinia enterocolitica |
1233 | - |
enzyme Yop51 DELTA162, catalytic domain | Yersinia enterocolitica |
Storage Stability | Organism |
---|---|
4°C, 100 mM acetate, pH 5.7, 1 mM EDTA, ionic strength 0.15, several months, no loss of activity | Yersinia enterocolitica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Yersinia enterocolitica | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, Yop51*DELTA162, catalytic domain, SDS-PAGE | Yersinia enterocolitica |
monomer | 1 * 51000, enzymes Yop51 and Yop51*, SDS-PAGE | Yersinia enterocolitica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.2 | 1440 | 4-nitrophenyl phosphate | value strongly dependent on ionic strength and pH | Yersinia enterocolitica |