Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Na/HEPES pH 8.0, 18% (w/v) poly(ethyleneglycol) 3350 | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | competitive inhibitor of the cofactor Mg2+ | Staphylococcus aureus | |
Mg2+ | uncompetitive inhibitor at high concentrations | Staphylococcus aureus | |
myo-inositol | non-competitive inhibition | Staphylococcus aureus | |
PO43- | competitive inhibition | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, contains three Mg2+ ions in the active site | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol phosphate + H2O | Staphylococcus aureus | - |
myo-inositol + phosphate | - |
? | |
myo-inositol phosphate + H2O | Staphylococcus aureus MSSA476 | - |
myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A1I9GET0 | - |
- |
Staphylococcus aureus MSSA476 | A0A1I9GET0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol phosphate + H2O | - |
Staphylococcus aureus | myo-inositol + phosphate | - |
? | |
myo-inositol phosphate + H2O | - |
Staphylococcus aureus MSSA476 | myo-inositol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMPase I | - |
Staphylococcus aureus |
inositol monophosphatase | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
81 | - |
Li+ | pH and temperature not specified in the publication | Staphylococcus aureus | |
88 | - |
Mg2+ | pH and temperature not specified in the publication | Staphylococcus aureus |