Cloned (Comment) | Organism |
---|---|
expressed in HEK-293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D104N | IMPA2 mutant, no activity | Mus musculus |
D93N | IMPA1 mutant, no activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus | |
additional information | the enzyme shows weaker or almost no activity when Mg2+ is replaced by Ca2+, Mn2+, Na+, or K+ (2 mM each) | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O55023 | IMPA1 | - |
Mus musculus | Q91UZ5 | IMPA2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain cortex | - |
Mus musculus | - |
cerebellum | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
hippocampus | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
olfactory bulb | - |
Mus musculus | - |
pancreas | - |
Mus musculus | - |
small intestine | - |
Mus musculus | - |
spleen | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | IMPA1 shows intermediate activity towards alpha-D-glucose 1-phosphate, whereas IMPA2 shows significantly lower affinity | Mus musculus | alpha-D-glucose + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | IMPA1 shows strong activity towards D-myo-inositol 1-phosphate, whereas IMPA2 shows significantly lower affinity | Mus musculus | D-myo-inositol + phosphate | - |
? | |
scyllo-inositol 1-phosphate + H2O | IMPA1 shows strong activity towards scyllo-inositol 1-phosphate, whereas IMPA2 shows almost no affinity | Mus musculus | scyllo-inositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
IMPA1 | - |
Mus musculus |
IMPA2 | - |
Mus musculus |
IMPase | - |
Mus musculus |
myo-inositol monophosphatase | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | IMPA1 | Mus musculus |
7.5 | 8 | IMPA2 | Mus musculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the activity of IMPA1 drops to 30% at pH 8.0 | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.7 | - |
IMPA1, in the presence of 2 mM MgCl2 | Mus musculus | Li+ | |
25 | - |
IMPA2, in the presence of 2 mM MgCl2 | Mus musculus | Li+ |