Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
expressed in Escherichia coli Origami B(DE3) cells | Archaeoglobus fulgidus |
expressed in Escherichia coli strain K-12 AG1 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LiCl | - |
Archaeoglobus fulgidus | |
LiCl | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus | |
1.02 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | 127% activity in the presence of 500 mM Li+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Archaeoglobus fulgidus |
28600 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O30298 | - |
- |
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl Super Q-650S column chromatography, phenyl-Sepharose column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Methanocaldococcus jannaschii |
Toyopearl Super Q-650S column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | - |
Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
2'-AMP + H2O | - |
Archaeoglobus fulgidus | adenosine + phosphate | - |
? | |
2'-phosphoadenosine 5'-phosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
2'-phosphoadenosine 5'-phosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
D-fructose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-fructose + phosphate | - |
? | |
D-fructose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-fructose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-glucose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-glucose + phosphate | - |
? | |
D-myo-inositol 1,4-bisphosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
D-myo-inositol 1,4-bisphosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Escherichia coli | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 2-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Archaeoglobus fulgidus | ? | - |
? | |
additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Archaeoglobus fulgidus | ? | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Methanocaldococcus jannaschii | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Archaeoglobus fulgidus | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanocaldococcus jannaschii |
dimer | - |
Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
AF2372 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to inositol monophosphatase activitity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Archaeoglobus fulgidus |
IMPase | - |
Escherichia coli |
IMPase | - |
Methanocaldococcus jannaschii |
IMPase | - |
Archaeoglobus fulgidus |
inositol phosphatase | - |
Escherichia coli |
inositol phosphatase | - |
Methanocaldococcus jannaschii |
inositol phosphatase | - |
Archaeoglobus fulgidus |
MJ0109 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to the inositol monophosphatase activity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Methanocaldococcus jannaschii |
SuhB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii | |
28.7 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
30 | - |
- |
Escherichia coli | LiCl | |
30 | - |
- |
Archaeoglobus fulgidus | LiCl |