Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not upregulated upon thermal stress | Archaeoglobus fulgidus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Methanocaldococcus jannaschii |
expression in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
S171A | site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows impaired Mg2+ binding, weak substrate binding at all temperatures, and no change in Km with increasing temperature in contrast to the wild-type enzyme | Archaeoglobus fulgidus |
T174L | site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows no change in Km with increasing temperature in contrast to the wild-type enzyme | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis | Methanocaldococcus jannaschii | |
additional information | - |
additional information | analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis | Thermotoga maritima | |
additional information | - |
additional information | analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, the enzyme's fructose bisphosphatase activity is temperature independent between 55°C and 70°C, but fructose bisphosphate is not stable above 70°C | Archaeoglobus fulgidus | |
1.67 | - |
L-myo-Inositol 1-phosphate | 85°C | Archaeoglobus fulgidus | |
13.2 | - |
L-myo-Inositol 1-phosphate | 75°C | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Methanocaldococcus jannaschii | |
Mg2+ | - |
Thermotoga maritima | |
Mg2+ | dependent on, involved in temeprature-dependent substrate affinity regulation | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus | - |
D-myo-inositol + phosphate | - |
? | |
L-myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus | - |
L-myo-inositol + phosphate | - |
? | |
additional information | Archaeoglobus fulgidus | at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity | ? | - |
? | |
additional information | Methanocaldococcus jannaschii | the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O30298 | - |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography | Methanocaldococcus jannaschii |
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography | Thermotoga maritima |
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography | Archaeoglobus fulgidus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
myo-inositol phosphate + H2O = myo-inositol + phosphate | the active site residues Tyr155, Glu175, Asp85, and Ser171 are required for the hydrogen bond network in the active site | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dideoxy-D-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | 3,5-dideoxy-D-myo-inositol + phosphate | - |
? | |
3,5-dideoxy-L-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | 3,5-dideoxy-L-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
L-myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | L-myo-inositol + phosphate | - |
? | |
L-myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima | L-myo-inositol + phosphate | - |
? | |
L-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | L-myo-inositol + phosphate | - |
? | |
additional information | at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity | Archaeoglobus fulgidus | ? | - |
? | |
additional information | the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | the enzyme also shows low fructose bisphosphatase activity | Archaeoglobus fulgidus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanocaldococcus jannaschii |
dimer | - |
Archaeoglobus fulgidus |
tetramer | - |
Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
IMPase | - |
Methanocaldococcus jannaschii |
IMPase | - |
Thermotoga maritima |
IMPase | - |
Archaeoglobus fulgidus |
inositol monophosphatase | - |
Methanocaldococcus jannaschii |
inositol monophosphatase | - |
Thermotoga maritima |
inositol monophosphatase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Methanocaldococcus jannaschii |
70 | - |
assay at | Thermotoga maritima |
85 | - |
or above | Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 85 | the activity increases with increasing temperature, temperature profile | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
3,5-dideoxy-L-myo-inositol 1-phosphate | pH 8.0, 85°C | Archaeoglobus fulgidus | |
additional information | - |
additional information | values extrapolated from Arrhenius plot | Thermotoga maritima | |
2.49 | - |
3,5-dideoxy-D-myo-inositol 1-phosphate | pH 8.0, 85°C | Archaeoglobus fulgidus | |
3.64 | - |
L-myo-Inositol 1-phosphate | pH 8.0, 85°C | Archaeoglobus fulgidus | |
4.32 | - |
D-myo-Inositol 1-phosphate | pH 8.0, 85°C | Archaeoglobus fulgidus | |
5.78 | - |
3,5-dideoxy-L-myo-inositol 1-phosphate | pH 8.0, 85°C | Archaeoglobus fulgidus | |
7.72 | - |
D-myo-Inositol 1-phosphate | pH 8.0, 85°C | Methanocaldococcus jannaschii | |
7.92 | - |
L-myo-Inositol 1-phosphate | pH 8.0, 85°C | Methanocaldococcus jannaschii | |
16.6 | - |
L-myo-Inositol 1-phosphate | pH 8.0, 85°C | Thermotoga maritima | |
104 | - |
D-myo-Inositol 1-phosphate | pH 8.0, 85°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanocaldococcus jannaschii |
8 | - |
assay at | Thermotoga maritima |
8 | - |
assay at | Archaeoglobus fulgidus |