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Literature summary for 3.1.3.25 extracted from

  • Gill, R.; Mohammed, F.; Badyal, R.; Coates, L.; Erskine, P.; Thompson, D.; Cooper, J.; Gore, M.; Wood, S.
    High-resolution structure of myo-inositol monophosphatase, the putative target of lithium therapy (2005), Acta Crystallogr. Sect. D, 61, 545-555.
    View publication on PubMed
Search for more literature for 3.1.3.25

Application

Application Comment Organism
pharmacology the enzyme is the putative target of lithium therapy Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in complex with Mg2+, room temperature, hanging drop vapour diffusion method, 0.002 ml protein solution containing 20 mg/ml protein in 20 mm Tris-HCl, pH 7.5, and 20 mM MgCl2, is mixed with 0.002 ml reservoir solution containing 0.1 M sodium acetate, 0.1 M sodium HEPES, pH 8.5, and 15% PEG 4000, X-ray diffraction structure determination and analysis at 1.4 A resolution Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Li+ binding structure, modelling of the lithium-inhibited enzyme-product complex Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, three Mg2+ are octahedrally coordinated at the active site of each of the two subunits involving residues E70, D90, I92, D93, and D220, detailed three-metal mechanism, overview Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus the enzyme is the key enzyme of the phosphatidylinositol signalling pathway and the putative target of the mood-stabilizing drug lithium ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus P20456
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by ion exchange chromatography, ammonium sulfate fractionation, and dialysis Bos taurus

Reaction

Reaction Comment Organism Reaction ID
myo-inositol phosphate + H2O = myo-inositol + phosphate the catalytic mechanism involves three Mg2+ ions, modeling of the reaction and the postreaction complex, active site structure, substrate binding structure, overview Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is the key enzyme of the phosphatidylinositol signalling pathway and the putative target of the mood-stabilizing drug lithium Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer structural comparison of subunits A and B, dimer interface structure, crystal structure, comparison to the structure of the human enzyme, overview Bos taurus

Synonyms

Synonyms Comment Organism
myo-inositol monophosphatase
-
 Bos taurus