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Literature summary for 3.1.3.2 extracted from

  • Huang, S.; Zhang, J.; Ma, Y.; Wei, S.; Huang, L.
    Characterization of an acid phosphatase responsible for hydrolysis of pyridoxal 5-phosphate in tobacco plants (2012), Plant Physiol. Biochem., 57, 114-119.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl phosphate competitive Nicotiana tabacum
ATP 1 mM, 29% residual activity, competitive Nicotiana tabacum
EDTA 1 mM, 62% residual activity Nicotiana tabacum
fluoride 1 mM, 56% residual activity Nicotiana tabacum
molybdate 1 mM, 5% residual activity Nicotiana tabacum
additional information not inhibitory: levamisole and tartrate Nicotiana tabacum
phosphate 1 mM, 20% residual activity, competitive Nicotiana tabacum
pyridoxal 1 mM, 20% residual activity, competitive Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 pyridoxal 5'-phosphate pH 5.5, 37°C Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 179% of initial activity Nicotiana tabacum
Mg2+ most effective dimetal ion for catalysis under saturating substrate concentrations, 1 mM, 215% of initial activity Nicotiana tabacum
Mn2+ 1 mM, 154% of initial activity Nicotiana tabacum
additional information a divalent metal ion is required for activity Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24900
-
 2 * 24900, SDS-PAGE Nicotiana tabacum
50000
-
 gel filtration Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1998
-
 pH 5.5, 37°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Nicotiana tabacum 4-nitrophenol + phosphate about 158% of the activity with pyridoxal 5'-phosphate ?
diphosphate + H2O
-
 Nicotiana tabacum 2 phosphate about 156% of the activity with pyridoxal 5'-phosphate ?
pyridoxal 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxal + phosphate
-
 ?
pyridoxamine 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxamine + phosphate about 50% of the activity with pyridoxal 5'-phosphate ?
pyridoxine 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxine + phosphate about 50% of the activity with pyridoxal 5'-phosphate ?

Subunits

Subunits Comment Organism
dimer 2 * 24900, SDS-PAGE Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Nicotiana tabacum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 citric acid-sodium citrate buffer, pH 5.5, fully stable at least for 120 min Nicotiana tabacum
50
-
 half-life around 50 min Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Nicotiana tabacum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 37°C for 2 h, more than 80% of total activities Nicotiana tabacum