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Literature summary for 3.1.3.2 extracted from
- High affinity of acid phosphatase encoded by PHO3 gene in Saccharomyces cerevisiae for thiamin phosphates (1990), Biochim. Biophys. Acta, 1037, 147-154.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | highly increases the Km for thiamin phosphate | Saccharomyces cerevisiae |  |
| phosphate | competitive inhibition of phosphate-repressible acid phosphatase. iinhibition 4-nitrophenyl phosphate hydrolysis and slight inhibition thiamin phosphate hydrolysis by thiamin-repressible acid phosphatase | Saccharomyces cerevisiae | |
| thiamin | competitive versus thiamin phosphate, slight inhibition 4-nitrophenyl phosphate hydrolysis. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide modification of the enzyme causes complete loss of thiamin binding activity. The thiamin binding of the thiamin-repressible acid phosphatase is optimal at pH 5.2 and lost at pH 3.0 | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
thiamine phosphate | thiamine-repressible acid phosphatase, pH 5.0, 37°C | Saccharomyces cerevisiae | |
| 0.0017 | - |
thiamine diphosphate | thiamine-repressible acid phosphatase, pH 5.0, 37°C | Saccharomyces cerevisiae | |
| 0.0028 | - |
thiamine diphosphate | thiamine-repressible acid phosphatase, pH 3.0, 37°C | Saccharomyces cerevisiae | |
| 0.0063 | - |
thiamine phosphate | thiamine-repressible acid phosphatase, pH 3.0, 37°C | Saccharomyces cerevisiae | |
| 0.2 | - |
thiamine diphosphate | phosphate-repressible acid phosphatase, pH 4.5, 37°C | Saccharomyces cerevisiae | |
| 0.22 | - |
4-nitrophenyl phosphate | phosphate-repressible acid phosphatase, pH 4.5, 37°C | Saccharomyces cerevisiae | |
| 0.25 | - |
4-nitrophenyl phosphate | thiamine-repressible acid phosphatase, pH 3.0, 37°C | Saccharomyces cerevisiae | |
| 0.27 | - |
thiamine phosphate | phosphate-repressible acid phosphatase, pH 4.5, 37°C | Saccharomyces cerevisiae | |
| 1.5 | - |
4-nitrophenyl phosphate | thiamine-repressible acid phosphatase, pH 5.0, 37°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasmic space | thiamin-repressible acid phosphatase | Saccharomyces cerevisiae | 42597 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, about, thiamine-repressible acid phosphatase, sequence calculation | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine diphosphate + 2 H2O | Saccharomyces cerevisiae | - |
thiamine + 2 phosphate | - |
? | |
| thiamine phosphate + H2O | Saccharomyces cerevisiae | - |
thiamine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P24031 | gene PH03 encoding thiamin-repressible acid phosphatase, gene PH05 encoding phosphate-repressible acid phosphatase | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is highly glycosylated | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native thiamine-repressible acid phosphatase by cold osmotic shock treatment, anion exchange chromatography, and ultrafiltration | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
purified thiamine-repressible acid phosphatase, substrate glucose 1-phosphate, pH 3.0, 37°C | Saccharomyces cerevisiae |
| 17.1 | - |
purified thiamine-repressible acid phosphatase, substrate thiazole phosphate, pH 3.0, 37°C | Saccharomyces cerevisiae |
| 61.3 | - |
purified thiamine-repressible acid phosphatase, substrate thiamine phosphate, pH 3.0, 37°C | Saccharomyces cerevisiae |
| 66.2 | - |
purified thiamine-repressible acid phosphatase, substrate ATP, pH 3.0, 37°C | Saccharomyces cerevisiae |
| 71.5 | - |
purified thiamine-repressible acid phosphatase, substrate 4-nitrophenyl phosphate, pH 3.0, 37°C | Saccharomyces cerevisiae |
| 221.2 | - |
purified thiamine-repressible acid phosphatase, substrate thiamine diphosphate, pH 3.0, 37°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + phosphate | - |
? | |
| 5-hydroxyethyl-4-methylthiazole phosphate + H2O | - |
Saccharomyces cerevisiae | 5-hydroxyethyl-4-methylthiazole + phosphate | - |
? | |
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | low activity | Saccharomyces cerevisiae | D-glucose + phosphate | - |
? | |
| additional information | the thiamin-repressible acid phosphatase shows no activity with CMP. The phosphate-repressible acid phosphatase shows a broad substrate specificity on phosphate esters | Saccharomyces cerevisiae | ? | - |
? | |
| thiamine diphosphate + 2 H2O | - |
Saccharomyces cerevisiae | thiamine + 2 phosphate | - |
? | |
| thiamine phosphate + H2O | - |
Saccharomyces cerevisiae | thiamine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, thiamine-repressible acid phosphatase, sequence calculation | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| orthophosphoric-monoester phosphohydrolase | - |
Saccharomyces cerevisiae |
| phosphate-repressible acid phosphatase | - |
Saccharomyces cerevisiae |
| Pi-rAPase | - |
Saccharomyces cerevisiae |
| T-rAPase | - |
Saccharomyces cerevisiae |
| thiamin-repressible acid phosphatase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
thiamine-repressible acid phosphatase | Saccharomyces cerevisiae |
| 4.5 | - |
assay at, phosphate-repressible acid phosphatase | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | activity range, thiamine-repressible acid phosphatase | Saccharomyces cerevisiae |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | the thiamin-repressible acid phosphatase is repressed by thiamine, but not by phosphate | down |
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