Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Clostridium perfringens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-fusion protein | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | - |
Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.84 | - |
4-nitrophenyl phosphate | pH 4.8, 37°C | Clostridium perfringens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM Mg2Cl2 enhances activity | Clostridium perfringens | |
NaF | inhibits activity | Clostridium perfringens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
calculated from cDNA | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Clostridium perfringens | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid phosphatase | - |
Clostridium perfringens |
CPE0201 | - |
Clostridium perfringens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium perfringens |
40 | - |
optimal activity | Clostridium perfringens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity drops rapidly by 78% at 50°C and by 84% at 55°C | Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Clostridium perfringens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.6 | 5.2 | enzyme retains only 27% of the optimal activity at pH 4.4, which is rapidly increased to 60% at pH 4.6. It retains 94% of optimal activity at pH 5.0, but the activity dramatically drops to 67% at pH 5.2 | Clostridium perfringens |
General Information | Comment | Organism |
---|---|---|
malfunction | A CPE0201 mutant does not grow in a minimal medium containing 4-nitrophenyl phosphate, while it shows normal growth when Na2HPO4 is added to the medium | Clostridium perfringens |
physiological function | enzyme is associated with the surface of the cell, where it may function to acquire inorganic phosphate from organic phosphomonoesters in acidic conditions, which could play an important role in the survival and growth of Clostridium perfringens in the host tissue | Clostridium perfringens |