Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.2 extracted from

  • Gargova, S.; Sariyska, M.; Angelov, A.; Stoilova, I.
    Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate (2006), Folia Microbiol. (Praha), 51, 541-545.
    View publication on PubMed
Search for more literature for 3.1.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.95
-
 4-nitrophenyl phosphate at pH 2.1 and 30°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger 307
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-100 gel filtration, DEAE-Sepharose CL 6B column chromatography, and CM-Sepharose CL 6B column chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.66
-
 culture liquide Aspergillus niger
361.3
-
 after 37.5fold purification Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 163% activity compared to 4-nitrophenyl phosphate Aspergillus niger 1-naphthol + phosphate
-
 ?
1-naphthyl phosphate + H2O 163% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 1-naphthol + phosphate
-
 ?
2-phosphoenolpyruvate + H2O 75.6% activity compared to 4-nitrophenyl phosphate Aspergillus niger enolpyruvate + phosphate
-
 ?
2-phosphoglycerate + H2O 80.9% activity compared to 4-nitrophenyl phosphate Aspergillus niger glycerate + phosphate
-
 ?
2-phosphoglycerate + H2O 80.9% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 100% activity Aspergillus niger 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 100% activity Aspergillus niger 307 4-nitrophenol + phosphate
-
 ?
D-glucose 6-phosphate + H2O 85.2% activity compared to 4-nitrophenyl phosphate Aspergillus niger D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 85.2% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 D-glucose + phosphate
-
 ?
dodecasodium phytate + H2O 245% activity compared to 4-nitrophenyl phosphate Aspergillus niger ? + phosphate
-
 ?
NADP+ + H2O 129% activity compared to 4-nitrophenyl phosphate Aspergillus niger NAD+ + phosphate
-
 ?
NADP+ + H2O 129% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 NAD+ + phosphate
-
 ?
phenylphosphate + H2O 134% activity compared to 4-nitrophenyl phosphate Aspergillus niger phenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
pH 2.1 optimum acid phosphatase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
66
-
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2 2.4
-
 Aspergillus niger