Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.2 extracted from

  • Makde, R.D.; Dikshit, K.; Kumar, V.
    Protein engineering of class-A non-specific acid phosphatase (PhoN) of Salmonella typhimurium: modulation of the pH-activity profile (2006), Biomol. Eng., 23, 247-251.
    View publication on PubMed
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
I78A mutant displays significantly higher (as high as 160%) enzymatic efficiency over a broad pH range of 3.0-9.0 Salmonella enterica subsp. enterica serovar Typhimurium
I78D mutant possesses nearly twice the specific activity at its optimum pH of 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
I78H mutant exhibits an alkaline shift in the pH-optimum by 1.5 pH points relative to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.08
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.08
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.11
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.12
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.14
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
0.25
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.32
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.47
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
0.55
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
1.8
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q71EB8
-
-
Salmonella enterica subsp. enterica serovar Typhimurium MD6001 Q71EB8
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, SP-Sepharose column chromatography, and phenyl-Sepharose column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium MD6001 p-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
bNSAP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
class-A bacterial non-specific acid phosphatases
-
 Salmonella enterica subsp. enterica serovar Typhimurium
PhoN
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
0.99
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
2 8 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.5
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.8
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
5.9
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
8.1
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
11.83
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
12.1
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
16.1
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
16.9
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
18.24
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
19.7
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
19.95
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
20.84
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
21.53
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
22.25
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
23.32
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
26.42
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
32.2
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
41.6
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 wild type enzyme and mutant enzyme I78A Salmonella enterica subsp. enterica serovar Typhimurium
7
-
 mutant enzymes I78D and I78H Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
 Salmonella enterica subsp. enterica serovar Typhimurium