Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.1-8.5, and 28-36% (w/v) PEG 4000 | Haemophilus influenzae |
Protein Variants | Comment | Organism |
---|---|---|
F48C | reduced phosphatase activity | Haemophilus influenzae |
N218Q | reduced phosphatase activity | Haemophilus influenzae |
Q39E | reduced phosphatase activity | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tungstate | - |
Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metalloenzyme with Mg2+ being the most likely metal ion in the crystalline recombinant enzyme | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl phosphate + H2O | - |
Haemophilus influenzae | 4-methylumbelliferol + phosphate | - |
? | |
nicotinamide mononucleotide + H2O | - |
Haemophilus influenzae | nicotinamide riboside + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Haemophilus influenzae | p-nitrophenol + phosphate | - |
? | |
phenyl phosphate + H2O | - |
Haemophilus influenzae | phenol + phosphate | - |
? | |
phosphotyrosine + H2O | - |
Haemophilus influenzae | tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
lipoprotein e acid phosphatase | - |
Haemophilus influenzae |
P4 | - |
Haemophilus influenzae |