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Literature summary for 3.1.3.2 extracted from
- Characterization of a novel acid phosphatase from embryonic axes of kidney bean exhibiting vanadate-dependent chloroperoxidase activity (2004), J. Biol. Chem., 279, 37477-37484.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tartrate | slight activation | Phaseolus vulgaris |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene KeACP, DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| molybdate | complete inhibition at 0.1 mM | Phaseolus vulgaris | |
| vanadate | 30 min, 30°C, leads to complete loss of phosphatase activity of the enzyme at 0.1 mM and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not affected by citrate, EDTA, fluoride, Zn2+, Fe3+, and Mn2+ | Phaseolus vulgaris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
| 56000 | - |
1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
| 96000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | Q764C1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | both subunit types are glycosylated | Phaseolus vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 852fold to homogeneity from embryonic axes by DEAE ion exchange and concanavalin A affinity chromatography | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | expression during elongation in germination | Phaseolus vulgaris | - |
| seed | embryonic axes, expression during elongation in germination | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 383.3 | - |
purified enzyme | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
| 3-phosphoglycerate + H2O | - |
Phaseolus vulgaris | glycerate + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Phaseolus vulgaris | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | low activity | Phaseolus vulgaris | AMP + phosphate | - |
? | |
| alpha-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | alpha-naphthol + phosphate | - |
? | |
| AMP + H2O | - |
Phaseolus vulgaris | adenosine + phosphate | - |
? | |
| ATP + H2O | - |
Phaseolus vulgaris | ADP + phosphate | - |
? | |
| beta-naphthyl phosphate + H2O | - |
Phaseolus vulgaris | beta-naphthol + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
| additional information | substrate specificity, overview, vanadate leads to loss of phosphatase activity of the enzyme and gain of chloroperoxidase activity by the enzyme, overview | Phaseolus vulgaris | ? | - |
? | |
| phospho-L-serine + H2O | low activity | Phaseolus vulgaris | L-serine + phosphate | - |
? | |
| phospho-L-threonine + H2O | - |
Phaseolus vulgaris | L-threonine + phosphate | - |
? | |
| phospho-L-tyrosine + H2O | - |
Phaseolus vulgaris | L-tyrosine + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Phaseolus vulgaris | pyruvate + phosphate | - |
? | |
| phytate + H2O | low activity | Phaseolus vulgaris | ? + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 45000 + 1 * 56000, SDS-PAGE | Phaseolus vulgaris |
| More | three combinations of the two subunit types occur with the 56 kDa subunit dominating, no intersubunit disulfide linkages | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| colorless acid phosphatase | - |
Phaseolus vulgaris |
| KeACP | - |
Phaseolus vulgaris |
| More | the colorless acid phosphatase is distinct from the purple acid phosphatase | Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Phaseolus vulgaris |
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