Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | poor inhibition by Zn2+, Mn2+, Mg2+, EDTA, and EGTA at 5 mM each | Landoltia punctata | |
phosphate | competitive, 54% inhibition at 5 mM | Landoltia punctata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Landoltia punctata |
120000 | - |
gel filtration and native PAGE | Landoltia punctata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Landoltia punctata | - |
aquatic duckweed | - |
Purification (Comment) | Organism |
---|---|
1871fold by ammonium sulfate precipitation, hydrophobic interaction and ion exchange chromatography, and gel filtration | Landoltia punctata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Landoltia punctata |
118 | - |
purified enzyme, substrate 4-nitrophenyl phosphate | Landoltia punctata |
125 | - |
purified enzyme, substrate ATP | Landoltia punctata |
131 | - |
purified enzyme, substrate diphosphate | Landoltia punctata |
156 | - |
purified enzyme, substrate AMP | Landoltia punctata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-dichlorovinyl dimethylphosphate + H2O | i.e. dichlorvos, an organophosphate pesticide, complete degradation | Landoltia punctata | ? | - |
? | |
3-phosphoglycerate + H2O | - |
Landoltia punctata | glycerate + phosphate | - |
? | |
4-methylumbelliferyl phosphate + H2O | - |
Landoltia punctata | 4-methylumbelliferol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Landoltia punctata | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Landoltia punctata | AMP + phosphate | - |
? | |
AMP + H2O | best substrate | Landoltia punctata | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Landoltia punctata | ADP + phosphate | - |
? | |
diphosphate + H2O | - |
Landoltia punctata | 2 phosphate | - |
? | |
additional information | no activity with the pesticide dylox | Landoltia punctata | ? | - |
? | |
O,O,O,O-tetraethyldithiodiphosphate + H2O | i.e. sulfatep, an organophosphate pesticide, 50% degradation | Landoltia punctata | ? | - |
? | |
O,O-dimethyl O-4-nitrophenyl phosphate + H2O | i.e. paraoxon, an organophosphate pesticide | Landoltia punctata | ? | - |
? | |
O-2-chloro-4-nitrophenyl O,O-dimethylphosphorothionate + H2O | i.e. dicapthon, an organophosphate pesticide, complete degradation | Landoltia punctata | ? | - |
? | |
phospho-L-serine + H2O | - |
Landoltia punctata | L-serine + phosphate | - |
? | |
S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethylphosphorodithionate + H2O | i.e. malathion, an organophosphate pesticide, 95% degradation | Landoltia punctata | ? | - |
? | |
S-2-ethylthioethyl O,O-dimethylphosphothionate + H2O | i.e. demeton-S-methyl, an organophosphate pesticide, 36% degradation | Landoltia punctata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, SDS-PAGE | Landoltia punctata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Landoltia punctata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | stable | Landoltia punctata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Landoltia punctata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 90% of maximal activity at pH 5.0, 20% at pH 8.0, substrate 4-nitrophenyl phosphate | Landoltia punctata |