Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-ascorbic acid | activates | Ipomoea batatas |
Cloned (Comment) | Organism |
---|---|
subcloning in Escherichia coli, expression of wild-type and mutant isozyme 3 in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Ipomoea batatas |
Protein Variants | Comment | Organism |
---|---|---|
Y291A | site-directed mutagenesis of an substrate binding ssite residue to the correspondent residue of isozyme 1, the mutant shows decreased activity compared to the wild-type isozyme 3 | Ipomoea batatas |
Y291H | site-directed mutagenesis of an substrate binding ssite residue to the correspondent residue of isozyme 1, the mutant shows increased activity compared to the wild-type isozyme 3 | Ipomoea batatas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Ipomoea batatas | |
Zn2+ | - |
Ipomoea batatas |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | isozyme 3 contains a diiron metal center in contrast to isozymes 1 and 2 which contain Fe(III)-Mn(II) centers, analysis of prosthetic metal ions/groups | Ipomoea batatas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, isozyme 3, SDS-PAGE | Ipomoea batatas |
110000 | - |
isozyme 3, gel filtration | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | Q9ZS50 | isozyme 3 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme 3 199fold from Sf9 insect cells in two steps by QBB-resin and hydrophobic interaction chromatography | Ipomoea batatas |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
321.8 | - |
purified recombinant wild-type enzyme | Ipomoea batatas |
545 | - |
purified recombinant wild-type enzyme in presence of L-ascorbic acid at 10 mM | Ipomoea batatas |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, isozyme 3, SDS-PAGE | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Ipomoea batatas |
purple acid phosphatase | - |
Ipomoea batatas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Ipomoea batatas |