Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 311% activity of isozyme II, no effect on isozyme I | Pinctada fucata | |
Cr3+ | 130% activity of isozyme I, no effect on isozyme II | Pinctada fucata | |
Fe2+ | 152% activity of isozyme II | Pinctada fucata | |
Mn2+ | 113% activity of isozyme II, 107% of isozyme I | Pinctada fucata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 92% inhibition of isozyme I and 45% inhibition of isozyme II at 5 mM | Pinctada fucata | |
EDTA | 21% inhibition of isozyme I and poor inhibition of isozyme II at 5 mM | Pinctada fucata | |
Fe2+ | 30% inhibition of isozyme I at 5 mM | Pinctada fucata | |
Fe3+ | strong inhibition of both isozymes at 5 mM | Pinctada fucata | |
fluoride | complete inhibition of both isozymes at 5 mM | Pinctada fucata | |
L-Tartrate | 95% inhibition of isozyme I and 77% inhibition of isozyme II at 20 mM | Pinctada fucata | |
additional information | no or poor inhibition of both isozymes by EGTA and Cd2+ | Pinctada fucata | |
Pb2+ | 54% inhibition of isozyme I and 41% inhibition of isozyme II at 5 mM | Pinctada fucata | |
tungstate | over 90% inhibition of both isozymes at 5 mM | Pinctada fucata | |
Zn2+ | 56% inhibition of isozyme I and 27% inhibition of isozyme II at 5 mM | Pinctada fucata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.194 | - |
4-nitrophenyl phosphate | pH 4.6, 47°C, purified isozyme I | Pinctada fucata | |
0.237 | - |
4-nitrophenyl phosphate | pH 3.2, 57°C, purified isozyme II | Pinctada fucata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29800 | - |
2 * 29800, isozyme II, SDS-PAGE | Pinctada fucata |
197100 | - |
isozyme I, gel filtration | Pinctada fucata |
208800 | - |
1 * 208800, isozyme I, SDS-PAGE | Pinctada fucata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinctada fucata | - |
pearl oyster, two isozymes AcPAse I and II | - |
Purification (Comment) | Organism |
---|---|
isozymes AcPAse I and II, 160.5fold and 613.6fold, respectively, from viscera to homogeneity by DEAE ion exchange chromatography, gel filtration, and concanavalin A affinity chromatography | Pinctada fucata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Pinctada fucata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
purified isozyme I | Pinctada fucata |
0.73 | - |
purified isozyme II | Pinctada fucata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + H2O | - |
Pinctada fucata | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | best substrate | Pinctada fucata | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Pinctada fucata | AMP + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | - |
Pinctada fucata | alpha-naphthol + phosphate | - |
? | |
AMP + H2O | best substrate | Pinctada fucata | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Pinctada fucata | ADP + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | - |
Pinctada fucata | D-fructose + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | - |
Pinctada fucata | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Pinctada fucata | D-glucose + phosphate | - |
? | |
additional information | isozymes I and II are non-specific and show a broad substrate spectrum, substrate specificties, overview | Pinctada fucata | ? | - |
? | |
phenyl phosphate + H2O | - |
Pinctada fucata | phenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29800, isozyme II, SDS-PAGE | Pinctada fucata |
monomer | 1 * 208800, isozyme I, SDS-PAGE | Pinctada fucata |
Synonyms | Comment | Organism |
---|---|---|
AcPase | - |
Pinctada fucata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
isozyme I | Pinctada fucata |
57 | - |
isozyme II | Pinctada fucata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
isozymes I and II are stable for 40 min | Pinctada fucata |
60 | - |
isozyme I shows 81% reduction of activity after 5 min, isozyme II shows 57% reduction of activity after 40 min | Pinctada fucata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
isozyme II | Pinctada fucata |
4.6 | - |
isozyme I | Pinctada fucata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.8 | 4.4 | isozyme II, 80% activity at pH 2.8 and pH 4.4 | Pinctada fucata |
4 | 5.2 | isozyme I, 80% activity at pH 4.0 and pH 5.2 | Pinctada fucata |