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Literature summary for 3.1.3.2 extracted from

  • Jing, G.; Li, L.; Li, Y.; Xie, L.; Zhang, R.
    Purification and partial characterization of two acid phosphatase forms from pearl oyster (Pinctada fucata) (2006), Comp. Biochem. Physiol. B, 143, 229-235.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Co2+ 311% activity of isozyme II, no effect on isozyme I Pinctada fucata
Cr3+ 130% activity of isozyme I, no effect on isozyme II Pinctada fucata
Fe2+ 152% activity of isozyme II Pinctada fucata
Mn2+ 113% activity of isozyme II, 107% of isozyme I Pinctada fucata

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 92% inhibition of isozyme I and 45% inhibition of isozyme II at 5 mM Pinctada fucata
EDTA 21% inhibition of isozyme I and poor inhibition of isozyme II at 5 mM Pinctada fucata
Fe2+ 30% inhibition of isozyme I at 5 mM Pinctada fucata
Fe3+ strong inhibition of both isozymes at 5 mM Pinctada fucata
fluoride complete inhibition of both isozymes at 5 mM Pinctada fucata
L-Tartrate 95% inhibition of isozyme I and 77% inhibition of isozyme II at 20 mM Pinctada fucata
additional information no or poor inhibition of both isozymes by EGTA and Cd2+ Pinctada fucata
Pb2+ 54% inhibition of isozyme I and 41% inhibition of isozyme II at 5 mM Pinctada fucata
tungstate over 90% inhibition of both isozymes at 5 mM Pinctada fucata
Zn2+ 56% inhibition of isozyme I and 27% inhibition of isozyme II at 5 mM Pinctada fucata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.194
-
4-nitrophenyl phosphate pH 4.6, 47°C, purified isozyme I Pinctada fucata
0.237
-
4-nitrophenyl phosphate pH 3.2, 57°C, purified isozyme II Pinctada fucata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29800
-
2 * 29800, isozyme II, SDS-PAGE Pinctada fucata
197100
-
isozyme I, gel filtration Pinctada fucata
208800
-
1 * 208800, isozyme I, SDS-PAGE Pinctada fucata

Organism

Organism UniProt Comment Textmining
Pinctada fucata
-
pearl oyster, two isozymes AcPAse I and II
-

Purification (Commentary)

Purification (Comment) Organism
isozymes AcPAse I and II, 160.5fold and 613.6fold, respectively, from viscera to homogeneity by DEAE ion exchange chromatography, gel filtration, and concanavalin A affinity chromatography Pinctada fucata

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
Pinctada fucata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.58
-
purified isozyme I Pinctada fucata
0.73
-
purified isozyme II Pinctada fucata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphoglycerate + H2O
-
Pinctada fucata glycerate + phosphate
-
?
4-nitrophenyl phosphate + H2O best substrate Pinctada fucata 4-nitrophenol + phosphate
-
?
ADP + H2O
-
Pinctada fucata AMP + phosphate
-
?
alpha-naphthyl phosphate + H2O
-
Pinctada fucata alpha-naphthol + phosphate
-
?
AMP + H2O best substrate Pinctada fucata adenosine + phosphate
-
?
ATP + H2O
-
Pinctada fucata ADP + phosphate
-
?
D-fructose 6-phosphate + H2O
-
Pinctada fucata D-fructose + phosphate
-
?
D-glucose 1-phosphate + H2O
-
Pinctada fucata D-glucose + phosphate
-
?
D-glucose 6-phosphate + H2O
-
Pinctada fucata D-glucose + phosphate
-
?
additional information isozymes I and II are non-specific and show a broad substrate spectrum, substrate specificties, overview Pinctada fucata ?
-
?
phenyl phosphate + H2O
-
Pinctada fucata phenol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29800, isozyme II, SDS-PAGE Pinctada fucata
monomer 1 * 208800, isozyme I, SDS-PAGE Pinctada fucata

Synonyms

Synonyms Comment Organism
AcPase
-
Pinctada fucata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
47
-
isozyme I Pinctada fucata
57
-
isozyme II Pinctada fucata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
isozymes I and II are stable for 40 min Pinctada fucata
60
-
isozyme I shows 81% reduction of activity after 5 min, isozyme II shows 57% reduction of activity after 40 min Pinctada fucata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2
-
isozyme II Pinctada fucata
4.6
-
isozyme I Pinctada fucata

pH Range

pH Minimum pH Maximum Comment Organism
2.8 4.4 isozyme II, 80% activity at pH 2.8 and pH 4.4 Pinctada fucata
4 5.2 isozyme I, 80% activity at pH 4.0 and pH 5.2 Pinctada fucata