Cloned (Comment) | Organism |
---|---|
stable expression of wild-type enzyme in CHO-K1 cells, expression of wild-type and mutant enzymes in Spodopetra frugiperda Sf9 cells using the baculovirus infection system | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
N128Q | site-directed mutagenesis, reduced N-glycosylation and slightly shifted pH optimum compared to the wild-type enzyme | Rattus norvegicus |
N97Q | site-directed mutagenesis, reduced N-glycosylation and slightly shifted pH optimum compared to the wild-type enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is tartrate-insensitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant wild-type and mutant enzymes | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | activates | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylated, detectable by Galanthus nivalis agglutinin | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
native enzyme from bone, recombinant enzyme from CHO-K1 cells, recombinant wild-type and mutant enzymes from Sf9 insect cells by cation exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and heparin affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Rattus norvegicus | - |
femur | - |
Rattus norvegicus | - |
tibia | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000-37000, SDS-PAGE | Rattus norvegicus |
More | cathepsin L proteolytically cleaved recombinant wild-type and mutant enzymes show two fragment of 16 and 23 kDa | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Rattus norvegicus |
purple acid phosphatase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | 5 | recombinant wild-type and mutant enzymes | Rattus norvegicus |
5.8 | 6 | cathepsin L proteolytically cleaved recombinant wild-type and mutant enzymes | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 6.7 | - |
Rattus norvegicus |