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Literature summary for 3.1.3.2 extracted from
- Porcine purple acid phosphatase: heterologous expression, characterization, and proteolytic analysis (2004), Arch. Biochem. Biophys., 432, 25-36.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | partial cleavage results in 1.7fold enzyme activation | Sus scrofa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Spodopetra frugiperda Sf9 cells using the baculovirus infection system, the recombinant enzyme is secreted from the cell culture | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Pepsin | partial cleavage results in enzyme inhibition | Sus scrofa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | two iron ions per enzyme molecule | Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
1 * 35000-37000 + 1 * 67000, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sus scrofa | the enzyme is an iron-binding protein required for maternal/fetal iron transport | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant enzyme | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant secreted enzyme 155fold from Sf9 insect cell culture expressing the enzyme by ion exchange, adsorption, and affinity chromatography, and gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| uterine endometrium | - |
Sus scrofa | - |
| uterus | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
recombinant enzyme in cell culture medium | Sus scrofa |
| 233 | - |
purified recombinant enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Sus scrofa | 4-nitrophenol + phosphate | - |
? | |
| additional information | the enzyme is an iron-binding protein required for maternal/fetal iron transport | Sus scrofa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 35000-37000 + 1 * 67000, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP | - |
Sus scrofa |
| purple acid phosphatase | - |
Sus scrofa |
| Uf | - |
Sus scrofa |
| uteroferrin | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Sus scrofa |
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