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Literature summary for 3.1.3.2 extracted from

  • Miyazaki, S.; Igarashi, M.; Nagata, A.; Komoda, T.
    Characterizations of recombinant human tartrate-resistant acid phosphatase from osteosarcoma: comparison study between recombinant and placental proteins (2001), Methods Find. Exp. Clin. Pharmacol., 23, 433-439.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates, enhancement of the enzyme activity by reducing agents might be expressed via the reduction of Fe2+ at the metal center Homo sapiens
ascorbic acid activates, enhancement of the enzyme activity by reducing agents might be expressed via the reduction of Fe2+ at the metal center Homo sapiens
dithiothreitol activates, enhancement of the enzyme activity by reducing agents might be expressed via the reduction of Fe2+ at the metal center Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Fe3+
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enhances activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
osteosarcoma cell
-
Homo sapiens
-
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
133.3
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
tartrate-resistant acid phosphatase
-
Homo sapiens
TRAP
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 MES buffer, recombinant enzyme Homo sapiens
6 6.5 MES buffer, enzyme from placenta Homo sapiens