Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrodictium abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pyrodictium abyssi | |
diethyldicarbonate | 0.5%. No inhibition at 0.05% and 0.1% | Pyrodictium abyssi | |
EDTA | - |
Pyrodictium abyssi | |
Fe2+ | - |
Pyrodictium abyssi | |
NaF | - |
Pyrodictium abyssi | |
okadaic acid | inhibition is greater at 40°C than at 90°C | Pyrodictium abyssi | |
sodium potassium tartrate | - |
Pyrodictium abyssi | |
Zn2+ | - |
Pyrodictium abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM | Pyrodictium abyssi | |
Mg2+ | activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation with Mg2+ is induced between 5 and 10 mM | Pyrodictium abyssi | |
Mn2+ | activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+ is the most effective divalent metal ion. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM | Pyrodictium abyssi | |
Ni2+ | activity is dependent on the presence of divalent ions such as Mn2+, Co2+, Ni2+, or Mg2+. Mn2+, Co2+, Ni2+ are much more effective than Mg2+. Maximal activation is induced at concentrations in the range of 0.5 to 1 mM | Pyrodictium abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33340 | - |
x * 33340, calculation from sequence | Pyrodictium abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrodictium abyssi | O08367 | - |
- |
Pyrodictium abyssi TAG11 | O08367 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrodictium abyssi |
Renatured (Comment) | Organism |
---|---|
the protein denatured by 6 M guanidinium chloride is refolded by dilution (100fold) into a buffer containing 0.5 M L-arginine | Pyrodictium abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a peptide]-serine/threonine phosphate + H2O | - |
Pyrodictium abyssi | [a peptide]-serine/threonine + phosphate | - |
? | |
[a peptide]-serine/threonine phosphate + H2O | - |
Pyrodictium abyssi TAG11 | [a peptide]-serine/threonine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33340, calculation from sequence | Pyrodictium abyssi |
Synonyms | Comment | Organism |
---|---|---|
protein-serine/threonine phosphatase | - |
Pyrodictium abyssi |
Py-PP1 | - |
Pyrodictium abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrodictium abyssi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 110 | the enzyme is active in the range from 40 to 110°C | Pyrodictium abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 180 min | Pyrodictium abyssi |
100 | - |
half-life: 7 min | Pyrodictium abyssi |
105 | - |
half-life: 3 min | Pyrodictium abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Pyrodictium abyssi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH changes (pH 4.5 to 8.0) have no significant effect on enzyme activity. No activity is observed below pH 4.0 or above 8.5 | Pyrodictium abyssi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrodictium abyssi | calculation from sequence | - |
6.15 |