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Literature summary for 3.1.3.16 extracted from

  • Hsu, J.L.; Chen, H.C.; Peng, H.L.; Chang, H.Y.
    Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1 (2008), J. Biol. Chem., 283, 9933-9944.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PA3346, DNA and amino acid sequence determination and analysis, genetic organization, reverse transcription-PCR analysis revealing an operon structure of hptB-PA3346–PA3347, coordinate expression of the three genes, overview Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information allelic exchange strategy is used to generate gene-specific mutants in Pseudomonas aeruginosa. Construction of a PA3346 gene knockout mutant with affected bacterial swarming activity and biofilm formation, overview Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB ?
-
?
phospho-PA3347 + H2O Pseudomonas aeruginosa dephosphorylation of Ser56 by PA3346 PA3347 + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
gene PA3346
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein PA3346 is phosphorylated in the active state by HptB Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
biofilm formation activity of wild-type and mutant enzymes, overview Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
-
Pseudomonas aeruginosa 6,8-difluoro-4-methylumbelliferone + phosphate
-
?
additional information the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB Pseudomonas aeruginosa ?
-
?
phospho-PA3347 + H2O dephosphorylation of Ser56 by PA3346 Pseudomonas aeruginosa PA3347 + phosphate
-
?

Subunits

Subunits Comment Organism
More the enzyme is a phosphatase consisting of an N-terminal receiver domain and a eukaryotic type Ser/Thr phosphatase domain Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
More PA3346 is a Ser/Thr phosphatase Pseudomonas aeruginosa
PA3346
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa