Cloned (Comment) | Organism |
---|---|
gene PA3346, DNA and amino acid sequence determination and analysis, genetic organization, reverse transcription-PCR analysis revealing an operon structure of hptB-PA3346PA3347, coordinate expression of the three genes, overview | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | allelic exchange strategy is used to generate gene-specific mutants in Pseudomonas aeruginosa. Construction of a PA3346 gene knockout mutant with affected bacterial swarming activity and biofilm formation, overview | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB | ? | - |
? | |
phospho-PA3347 + H2O | Pseudomonas aeruginosa | dephosphorylation of Ser56 by PA3346 | PA3347 + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene PA3346 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PA3346 is phosphorylated in the active state by HptB | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
biofilm formation activity of wild-type and mutant enzymes, overview | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,8-difluoro-4-methylumbelliferyl phosphate + H2O | - |
Pseudomonas aeruginosa | 6,8-difluoro-4-methylumbelliferone + phosphate | - |
? | |
additional information | the enzyme is involved in bacterial swarming activity and biofilm formation, PA3346 is the only of seven orphan response regulators inthe organism that is trans-phosphorylated by HptB | Pseudomonas aeruginosa | ? | - |
? | |
phospho-PA3347 + H2O | dephosphorylation of Ser56 by PA3346 | Pseudomonas aeruginosa | PA3347 + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is a phosphatase consisting of an N-terminal receiver domain and a eukaryotic type Ser/Thr phosphatase domain | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
More | PA3346 is a Ser/Thr phosphatase | Pseudomonas aeruginosa |
PA3346 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |