Cloned (Comment) | Organism |
---|---|
co-expression of the intein-enzyme fusion protein and the regulatory 34 kDa myosin phosphatase targeting subunit MYPT1 residues 1-299 in Escherichia coli strain BL21(DE3) | Gallus gallus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme bound to the regulatory 34 kDa myosin phosphatase targeting subunit MYPT1 residues 1-299, 8 mg/ml protein in 10 mM Tris, pH 7.5, 20 mM NaCl, 4 mM DTT, and 1 mM MnCl2, 20°C, hanging drop vapour diffusion method, mixed with an equal volume of reservoir solution containing 9.2% w/v PEG 5000 monomethylether, 35% v/v glycerol, and 200 mM NH4Cl, X-ray diffraction structure determination and analysis at 2.7 A resolution | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for full activiy | Gallus gallus | |
additional information | one of the two metal ions in the metal center of the enzyme might as well be Fe2+ instead of Mn2+ | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gallus gallus | the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P62207 | isozyme delta; PP1 isozyme delta | - |
Purification (Comment) | Organism |
---|---|
recombinant intein-enzyme fusion protein from Escherichia coli strain BL21(DE3) by chitin affinity chromatography, DTT-induced self-cleavage of the intein tag, followed by ion exchange chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
smooth muscle | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview | Gallus gallus | ? | - |
? | |
additional information | myosin phosphatase targeting subunit MYPT1 increases myosine specificity of PP1, interaction and binding structure, overview | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PP1 | - |
Gallus gallus |
protein phosphatase 1 | - |
Gallus gallus |