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Literature summary for 3.1.3.16 extracted from
- Suppressive effect of regucalcin on protein phosphatase activity in the heart cytosol of normal and regucalcin transgenic rats (2004), Int. J. Mol. Med., 13, 289-293.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of regucalcin-expressing transgenic Sprague-Dawley rats showing reduced protein phosphatase activity in the heart cytosol, overview | Rattus norvegicus |
| additional information | construction of transgenic rats expressing the inhibitory protein regucalcin in the heart muscle cell cytosol leading to reduced enzyme activity and abolishing the activating effect of Ca2+, overview | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| regucalcin | has regulatory function in heart cytosol; the protein inhibits the enzyme in presence of EGTA or ethylene glycol and has a regulatory function for the enzyme independently of Ca2+ | Rattus norvegicus | |
| trifluoperazin | cocmplete inhibition at 0.001-0.002 mM | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Rattus norvegicus | |
| Ca2+ | activates, synergistic with calmodulin, activation is completely inhibited by trifluoroperazine | Rattus norvegicus | |
| Calmodulin | activates the enzyme with substratees phospho-L-serine and phospho-L-threonine, but not with phospho-L-tyrosine | Rattus norvegicus | |
| Mg2+ | activates | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospho-L-serine + H2O | Rattus norvegicus | - |
L-serine + phosphate | - |
? | |
| phospho-L-threonine + H2O | Rattus norvegicus | - |
L-threonine + phosphate | - |
? | |
| phospho-L-tyrosine + H2O | Rattus norvegicus | preferred substrate | L-tyrosine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
female and male Wistar rats | - |
| Rattus norvegicus | - |
regucalcin-expressing transgenic Sprague-Dawley rats and wild-type male Wistar rats | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac muscle | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospho-L-serine + H2O | - |
Rattus norvegicus | L-serine + phosphate | - |
? | |
| phospho-L-threonine + H2O | - |
Rattus norvegicus | L-threonine + phosphate | - |
? | |
| phospho-L-tyrosine + H2O | - |
Rattus norvegicus | L-tyrosine + phosphate | - |
? | |
| phospho-L-tyrosine + H2O | preferred substrate | Rattus norvegicus | L-tyrosine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cf. EC 3.1.3.48, protein-tyrosine phosphatase | Rattus norvegicus |
| More | cf. EC 3.1.3.48, protein-tyrosine-phosphatase | Rattus norvegicus |
| protein phosphatase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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