Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged wild-type and mutant Wip1 catalytic subunits in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by okadaic acid | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, determination of dephosphorylation site-specific kinetic parameters for substrates p38alpha and UNG2, steady-state kinetics for wild-type and mutant p38alpha proteins, overview | Homo sapiens | |
0.036 | - |
ERK5 | pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylated p38alpha + H2O | Homo sapiens | mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway | p38alpha + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme PP2Cdelta | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and mutant Wip1 catalytic subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of isozyme PP2Cdelta, overview | Homo sapiens | ? | - |
? | |
phosphorylated ERK5 + H2O | dephosphorylation at Thr218 and Tyr220 residues, dephosphorylation site sequences, overview | Homo sapiens | ERK5 + phosphate | - |
? | |
phosphorylated JNK1 + H2O | dephosphorylation at Thr183 and Tyr185 residues, dephosphorylation site sequences, overview | Homo sapiens | JNK1 + phosphate | - |
? | |
phosphorylated p38alpha + H2O | mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway | Homo sapiens | p38alpha + phosphate | - |
? | |
phosphorylated p38alpha + H2O | substrate is a MAPK, identification of dephosphorylation at Thr residues, dephosphorylation site sequences, activity with wild-type and mutant p38alpha proteins, overview | Homo sapiens | p38alpha + phosphate | - |
? | |
phosphorylated peptide + H2O | PP2Cdelta prefers the dephosphorylation at diphosphorylated T(P)X(P)Y sequence compared to monophosphorylated T(P)XY sequence | Homo sapiens | peptide + phosphate | - |
? | |
phosphorylated UNG2 + H2O | dephosphorylation at Thr residues, identification of dephosphorylation site sequences, overview | Homo sapiens | UNG2 + phosphate | - |
? | |
RRAT(P)VA + H2O | synthetic substrate | Homo sapiens | RRATVA + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | EC 3.1.3.48, protein-tyrosine-phosphatase | Homo sapiens |
protein phosphatase 2Cdelta | - |
Homo sapiens |
wild-type p53-induced phosphatase | - |
Homo sapiens |
Wip1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
ERK5 | pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |