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Literature summary for 3.1.3.16 extracted from

  • Yamaguchi, H.; Minopoli, G.; Demidov, O.N.; Chatterjee, D.K.; Anderson, C.W.; Durell, S.R.; Appella, E.
    Substrate specificity of the human protein phosphatase 2Cdelta, Wip1 (2005), Biochemistry, 44, 5285-5294.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged wild-type and mutant Wip1 catalytic subunits in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by okadaic acid Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, determination of dephosphorylation site-specific kinetic parameters for substrates p38alpha and UNG2, steady-state kinetics for wild-type and mutant p38alpha proteins, overview Homo sapiens
0.036
-
ERK5 pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphorylated p38alpha + H2O Homo sapiens mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway p38alpha + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme PP2Cdelta
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and mutant Wip1 catalytic subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of isozyme PP2Cdelta, overview Homo sapiens ?
-
?
phosphorylated ERK5 + H2O dephosphorylation at Thr218 and Tyr220 residues, dephosphorylation site sequences, overview Homo sapiens ERK5 + phosphate
-
?
phosphorylated JNK1 + H2O dephosphorylation at Thr183 and Tyr185 residues, dephosphorylation site sequences, overview Homo sapiens JNK1 + phosphate
-
?
phosphorylated p38alpha + H2O mediation of a negative feedback loop of the p38 MAPK-p53 signaling pathway Homo sapiens p38alpha + phosphate
-
?
phosphorylated p38alpha + H2O substrate is a MAPK, identification of dephosphorylation at Thr residues, dephosphorylation site sequences, activity with wild-type and mutant p38alpha proteins, overview Homo sapiens p38alpha + phosphate
-
?
phosphorylated peptide + H2O PP2Cdelta prefers the dephosphorylation at diphosphorylated T(P)X(P)Y sequence compared to monophosphorylated T(P)XY sequence Homo sapiens peptide + phosphate
-
?
phosphorylated UNG2 + H2O dephosphorylation at Thr residues, identification of dephosphorylation site sequences, overview Homo sapiens UNG2 + phosphate
-
?
RRAT(P)VA + H2O synthetic substrate Homo sapiens RRATVA + phosphate
-
?

Synonyms

Synonyms Comment Organism
More EC 3.1.3.48, protein-tyrosine-phosphatase Homo sapiens
protein phosphatase 2Cdelta
-
Homo sapiens
wild-type p53-induced phosphatase
-
Homo sapiens
Wip1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
ERK5 pH 7.5, 30°C, recombinant His-tagged Wip1 catalytic subunit Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens