Literature summary for 3.1.3.16 extracted from

Shankar, S.; Kavanaugh-Black, A.; Kamath, S.; Chakrabarty, A.M.
Characterization of a phosphoprotein phosphatase for the phosphorylated form of nucleoside-diphosphate kinase from Pseudomonas aeruginosa (1995), J. Biol. Chem., 270, 28246-28250.

Search for more literature for 3.1.3.16

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	0.1 mM, complete inactivation	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+	Pseudomonas aeruginosa
Mn2+	metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+	Pseudomonas aeruginosa
Na+	metal required for activity. Order of decreasing efficiency: Mg2+, Mn2+, Na+	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphorylated nucleoside-diphosphate kinase + H2O	Pseudomonas aeruginosa	dephosphorylation of nucleoside-diphosphate kinase leads to loss of enzymatic activity, an efficient mode of posttranslational modification of the enzyme crucial to cellular development. Decreasing order of efficiency: nucleoside-diphosphate kinase from Homo sapiens, nucleoside-diphosphate kinase from Pseudomonas aeruginosa, nucleoside-diphosphate kinase from Escherichia coli, nucleoside-diphosphate kinase from yeast	nucleoside-diphosphate kinase + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.13	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with p-nitrophenyl phosphate	Pseudomonas aeruginosa	?	-	?
phosphorylated alpha-subunit of succinyl-CoA synthetase + H2O	activity is lower than towards phosphorylated nucleoside-diphosphate kinase	Pseudomonas aeruginosa	alpha-subunit of succinyl-CoA synthetase + phosphate	-	?
phosphorylated chemotaxis protein CheA + H2O	activity is lower than towards phosphorylated nucleoside-diphosphate kinase	Pseudomonas aeruginosa	chemotaxis protein CheA + phosphate	-	?
phosphorylated nucleoside-diphosphate kinase + H2O	-	Pseudomonas aeruginosa	nucleoside-diphosphate kinase + phosphate	-	?
phosphorylated nucleoside-diphosphate kinase + H2O	dephosphorylation of nucleoside-diphosphate kinase leads to loss of enzymatic activity, an efficient mode of posttranslational modification of the enzyme crucial to cellular development. Decreasing order of efficiency: nucleoside-diphosphate kinase from Homo sapiens, nucleoside-diphosphate kinase from Pseudomonas aeruginosa, nucleoside-diphosphate kinase from Escherichia coli, nucleoside-diphosphate kinase from yeast	Pseudomonas aeruginosa	nucleoside-diphosphate kinase + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
NPP	-	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
21	-	stable at room temperature for 2 h	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)