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Literature summary for 3.1.3.16 extracted from

  • de la Fuente van Bentem, S.; Vossen, J.H.; Vermeer, J.E.; de Vroomen, M.J.; Gadella, T.W., Jr.; Haring, M.A.; Cornelissen, B.J.
    The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing (2003), Plant Physiol., 133, 702-712.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
arachidonic acid
-
Solanum lycopersicum
linoleic acid
-
Solanum lycopersicum
linolenic acid
-
Solanum lycopersicum
oleic acid
-
Solanum lycopersicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus
expression in Escherichia coli Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
additional information removal of the TPR domain leads to a higher specific activity than wild-type enzyme at pH 7.5 but not at pH 5 Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
okadaic acid IC50: 0.0081 mM Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the 55000 Da isoform Solanum lycopersicum 5737
-
endoplasmic reticulum the 63000 Da isoform Solanum lycopersicum 5783
-
membrane the 63000 Da isoform, integral membran protein Solanum lycopersicum 16020
-
nucleus the 55000 Da isoform Solanum lycopersicum 5634
-
soluble the 55000 Da isoform Solanum lycopersicum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ when p-nitrophenyl phosphate is used as substrate 10 mM MgCl2 increases the activity, when phosphocasein is used as substrate 10 mM MgCl2 reduces the activity by 50% Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
Solanum lycopersicum
63000
-
-
Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P53042
-
-
Solanum lycopersicum Q84K11 tomato
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
at pH 7.5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum
0.034
-
at pH 5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum
0.064
-
at pH 5 with p-nitrophenyl phosphate as substrate Rattus norvegicus
0.081
-
at pH 7.5 with p-nitrophenyl phosphate as substrate Rattus norvegicus
0.245
-
DELTATPR at pH 7.5 with p-nitrophenyl phosphate as substrate Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
Rattus norvegicus 4-nitrophenol + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Solanum lycopersicum 4-nitrophenol + phosphate
-
?
phosphorylated casein + H2O
-
Rattus norvegicus casein + phosphate
-
?
phosphorylated casein + H2O
-
Solanum lycopersicum casein + phosphate
-
?

Synonyms

Synonyms Comment Organism
LePP5
-
Solanum lycopersicum
PP5
-
Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0081
-
IC50: 0.0081 mM Solanum lycopersicum okadaic acid