Literature summary for 3.1.3.16 extracted from

Stubbs, M.D.; Tran, H.T.; Atwell, A.J.; Smith, C.S.; Olson, D.; Moorhead, G.B.
Purification and properties of Arabidopsis thaliana type 1 protein phosphatase (PP1) (2001), Biochim. Biophys. Acta, 1550, 52-63.

Search for more literature for 3.1.3.16

Inhibitors

Inhibitors	Comment	Organism	Structure
calyculin	IC50: 0.09 nM	Arabidopsis thaliana
cantharidin	IC50: 60 nM	Arabidopsis thaliana
diphosphate	IC50: 0.07 mM	Arabidopsis thaliana
fostriecin	IC50: 0.022 mM	Arabidopsis thaliana
microcystin-LR	IC50: 0.01 nM	Arabidopsis thaliana
NaF	IC50: 2.1 mM	Arabidopsis thaliana
nodularin	IC50: 0.04 nM	Arabidopsis thaliana
okadaic acid	IC50: 20 nM	Arabidopsis thaliana
phosphate	IC50: 9.5 mM	Arabidopsis thaliana
protein phosphatase inhibitor-2	IC50: 0.65 nM	Arabidopsis thaliana
tautomycin	IC50: 0.06 nM	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	1 * 35000 Da, SDS-PAGE	Arabidopsis thaliana
39000	-	gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of the catalytic and the regulatory subunit	Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.95	-	-	Arabidopsis thaliana

Storage Stability

Storage Stability	Organism
-20°C, at least 2 months	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphorylated glycogen phosphorylase alpha + H2O	-	Arabidopsis thaliana	glycogen phosphorylase alpha + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 35000 Da, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
PP1	-	Arabidopsis thaliana

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00000001	-	IC50: 0.01 nM	Arabidopsis thaliana	microcystin-LR
0.00000004	-	IC50: 0.04 nM	Arabidopsis thaliana	nodularin
0.00000006	-	IC50: 0.06 nM	Arabidopsis thaliana	tautomycin
0.00000009	-	IC50: 0.09 nM	Arabidopsis thaliana	calyculin
0.00000065	-	IC50: 0.65 nM	Arabidopsis thaliana	protein phosphatase inhibitor-2
0.00002	-	IC50: 20 nM	Arabidopsis thaliana	okadaic acid
0.00006	-	IC50: 60 nM	Arabidopsis thaliana	cantharidin
0.022	-	IC50: 0.022 mM	Arabidopsis thaliana	fostriecin
0.07	-	IC50: 0.07 mM	Arabidopsis thaliana	diphosphate
2.1	-	IC50: 2.1 mM	Arabidopsis thaliana	NaF
9.5	-	IC50: 9.5 mM	Arabidopsis thaliana	phosphate

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Release 2023.1 (January 2023)