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Literature summary for 3.1.3.16 extracted from
- Characterisation of two protein phosphatase 2A holoenzymes from maize seedlings (2000), Biochim. Biophys. Acta, 1480, 65-76.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| histone H1 | increased activity | Zea mays | |
| polylysine | increased activity | Zea mays |  |
| protamine | in the presence of ammonium sulfate | Zea mays | |
| protamine | in the presence of ammoniumsulfate | Zea mays | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| okadaic acid | IC50: 0.18 nM; IC50: 0.1 nM | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE | Zea mays |
| 57000 | - |
alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE | Zea mays |
| 57000 | - |
x * 57000 + x * 66000, SDS-PAGE | Zea mays |
| 66000 | - |
alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE | Zea mays |
| 66000 | - |
x * 57000 + x * 66000, SDS-PAGE | Zea mays |
| 140000 | 160000 | gel filtration | Zea mays |
| 180000 | 200000 | gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.064 | - |
after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate | Zea mays |
| 0.12 | - |
after thiophosphorylase alpha-Sepharose purification step, with phosphorylated alpha phosphorylase as substrate and in the presence of 0.03 mg protamine and 16 mM ammonium sulfate | Zea mays |
| 0.49 | - |
with p-nitrophenyl phosphate as substrate | Zea mays |
| 1.12 | - |
with p-nitrophenyl phosphate as substrate | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl phosphate + H2O | - |
Zea mays | p-nitrophenol + phosphate | - |
? | |
| phosphorylated phosphorylase alpha + H2O | - |
Zea mays | phosphorylase alpha + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000 + x * 66000, SDS-PAGE | Zea mays |
| trimer | alphabetagamma, 1 * 66000 + 1 * 57000 + 1 * 38000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PP2AI | - |
Zea mays |
| PP2AII | - |
Zea mays |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000001 | - |
IC50: 0.1 nM | Zea mays | okadaic acid | |
| 0.00000018 | - |
IC50: 0.18 nM | Zea mays | okadaic acid | |
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Release 2023.1 (January 2023)
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