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Literature summary for 3.1.3.16 extracted from

  • Myles, T.; Schmidt, K.; Evans, D.R.H.; Cron, P.; Hemmings, B.A.
    Active-site mutations impairing the catalytic function of the catalytic subunit of human protein phosphatase 2A permit baculovirus-mediated overexpression in insect cells (2001), Biochem. J., 357, 225-232.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression in insect cells with the baculovirus system Homo sapiens

Protein Variants

Protein Variants Comment Organism
D88N reduction of specific activity in vitro, no function in vivo Homo sapiens
H118N reduction of specific activity in vitro, no function in vivo Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 RRApTVA 30°C, pH 7.5, D88n Homo sapiens
0.338
-
 RRApTVA 30°C, pH 7.5, wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RRApTVA + H2O
-
 Homo sapiens RRATVA + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 RRApTVA 30°C, pH 7.5, D88N Homo sapiens
170
-
 RRApTVA 30°C, pH 7.5, wild-type Homo sapiens