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Literature summary for 3.1.3.11 extracted from

  • Jang, H.; Cho, M.; Kwon, Y.; Bhoo, S.H.; Jeon, J.; Hahn, T.
    Roles of the residues Lys115 and Tyr116 in the binding of an allosteric inhibitor AMP to pea cytosolic D-fructose-1,6-bisphosphatase (2008), J. Appl. Biol. Chem., 51, 45-49.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Pisum sativum

Protein Variants

Protein Variants Comment Organism
K115Q Km (D-fructose 1,6-bisphosphate) and Ki (D-fructose 2,6-bisphosphate) comparable to wild-type, IC50 (AMP): 5.8 mM Pisum sativum
Y116Q Km (D-fructose 1,6-bisphosphate) and Ki (D-fructose 2,6-bisphosphate) comparable to wild-type, IC50 (AMP): 15.9 mM Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Pisum sativum
D-fructose 1,6-bisphosphate
-
Pisum sativum
D-fructose-2,6-bisphosphate
-
Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
D-fructose 1,6-bisphosphate mutant Y116F Pisum sativum
0.0107
-
D-fructose 1,6-bisphosphate mutant K115Q Pisum sativum
0.0108
-
D-fructose 1,6-bisphosphate wild-type Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Pisum sativum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Pisum sativum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.1
-
wild-type protein, after Ni-NTA chromatography Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Pisum sativum D-fructose 6-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Pisum sativum

Synonyms

Synonyms Comment Organism
D-fructose 1,6-bisphosphatase
-
Pisum sativum
FBPase
-
Pisum sativum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
D-fructose 1,6-bisphosphate mutant K115Q Pisum sativum
0.014
-
D-fructose 1,6-bisphosphate mutant Y116F Pisum sativum
0.015
-
D-fructose 2,6-bisphosphate wild-type Pisum sativum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
wild-type Pisum sativum AMP
5.81
-
mutant K115Q Pisum sativum AMP
15.96
-
mutant Y116F Pisum sativum AMP