Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.11 extracted from

  • Shyur, L.F.; Poland, B.W.; Honzatko, R.B.; Fromm, H.J.
    Major changes in the kinetic mechanism of AMP inhibition and AMP cooperativity attends the mutation of Arg49 in fructose-1,6-bisphosphatase (1997), J. Biol. Chem., 272, 26295-26299.
    View publication on PubMed
Search for more literature for 3.1.3.11

Protein Variants

Protein Variants Comment Organism
R49C less thermostable than wild type enzyme, wild type values for turnover number and Km-value Sus scrofa
R49D less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to uncompetitive. Loss of AMP cooperativity Sus scrofa
R49L less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Loss of AMP cooperativity Sus scrofa
R49M enzyme is more thermostable than wild type enzyme, kinetic properties are similar to the wild type enzyme. Loss of AMP cooperativity Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00344
-
 fructose 1,6-diphosphate mutant enzyme R49L Sus scrofa
0.00351
-
 fructose 1,6-diphosphate wild type enzyme Sus scrofa
0.00402
-
 fructose 1,6-diphosphate mutant enzyme K50M Sus scrofa
0.00407
-
 fructose 1,6-diphosphate mutant enzyme R49C Sus scrofa
0.00442
-
 fructose 1,6-diphosphate mutant enzyme R49D Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-diphosphate + H2O
-
 Sus scrofa D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18
-
 fructose 1,6-diphosphate wild type enzyme Sus scrofa
20.1
-
 fructose 1,6-diphosphate mutant enzyme R49D Sus scrofa
22.4
-
 fructose 1,6-diphosphate mutant enzyme K50M Sus scrofa
23.6
-
 fructose 1,6-diphosphate mutant enzyme R49L and R49C Sus scrofa