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Literature summary for 3.1.3.11 extracted from

  • Kurbanov, F.T.; Choe, J.; Honzatko, R.B.; Fromm, H.J.
    Directed mutations in the poorly defined region of porcine liver fructose-1,6-bisphosphatase significantly affect catalysis and the mechanism of AMP inhibition (1998), J. Biol. Chem., 273, 17511-17516.
    View publication on PubMed
Search for more literature for 3.1.3.11

Protein Variants

Protein Variants Comment Organism
D68E mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme Sus scrofa
D74A 50000fold reduced turnover number relative to wild type enzyme Sus scrofa
D74E mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive Sus scrofa
D74N 2000fold reduced turnover number relative to wild type enzyme Sus scrofa
K71A mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme Sus scrofa
K71M/K72M mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme, 175fold increased inhibition constant for AMP, 2fold increased affinity for Mg2+ Sus scrofa
N64A mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive Sus scrofa
N64Q mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00103
-
 fructose 1,6-diphosphate mutant enzyme D74E, pH 8.5 Sus scrofa
0.00131
-
 fructose 1,6-diphosphate mutant enzyme D68E, pH 7.5 Sus scrofa
0.00142
-
 fructose 1,6-diphosphate mutant enzyme N64A, pH 8.5 Sus scrofa
0.0016
-
 fructose 1,6-diphosphate mutant enzyme K71M/K72M, pH 7.5 Sus scrofa
0.0017
-
 fructose 1,6-diphosphate mutant enzyme N64E, pH 8.5 Sus scrofa
0.0022
-
 fructose 1,6-diphosphate wild type enzyme, pH 8.5 Sus scrofa
0.0028
-
 fructose 1,6-diphosphate wild type enzyme, pH 7.5 Sus scrofa
0.0028
-
 fructose 1,6-diphosphate mutant enzyme K71A, pH 7.5 Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-diphosphate + H2O
-
 Sus scrofa D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 fructose 1,6-diphosphate mutant enzyme D74A, pH 8.5 Sus scrofa
0.01
-
 fructose 1,6-diphosphate mutant enzyme D74N, pH 8.5 Sus scrofa
1.2
-
 fructose 1,6-diphosphate mutant enzyme D74E, pH 8.5 Sus scrofa
5.4
-
 fructose 1,6-diphosphate mutant enzyme N64E, pH 8.5 Sus scrofa
6.4
-
 fructose 1,6-diphosphate mutant enzyme D68E, pH 7.5 Sus scrofa
7.1
-
 fructose 1,6-diphosphate mutant enzyme N64A, pH 8.5 Sus scrofa
9.1
-
 fructose 1,6-diphosphate wild type enzyme, pH 8.5 Sus scrofa
16
-
 fructose 1,6-diphosphate mutant enzyme K71A, pH 7.5 Sus scrofa
21
-
 fructose 1,6-diphosphate wild type enzyme, pH 7.5 Sus scrofa
34
-
 fructose 1,6-diphosphate mutant enzyme K71M/K72M, pH 7.5 Sus scrofa