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Literature summary for 3.1.3.11 extracted from
- Hexosediphosphatase from spinach chloroplasts. Purification, crystallization and some properties (1974), Biochim. Biophys. Acta, 333, 366-377.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cAMP | slight activation | Spinacia oleracea |  |
| Ferredoxin | activates | Spinacia oleracea | |
| Gly | activates | Spinacia oleracea | |
| HCO3- | activates | Spinacia oleracea | |
| NH4+ | activates | Spinacia oleracea | |
| sulfhydryl reagents | activate | Spinacia oleracea | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MgADP- | - |
Spinacia oleracea | |
| MgATP2- | - |
Spinacia oleracea | |
| MgP2O42- | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Spinacia oleracea | |
| Mn2+ | can partially replace Mg2+ in activation | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for over 6 months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Spinacia oleracea | D-fructose 6-phosphate + phosphate | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | no activity | Spinacia oleracea | sedoheptulose 7-phosphate + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
at 40 mM Mg2+ | Spinacia oleracea |
| 8.5 | - |
in presence of 1-5 mM Mg2+ | Spinacia oleracea |
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Release 2023.1 (January 2023)
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